Unlock instant, AI-driven research and patent intelligence for your innovation.

Variable region gene of high neutralization activity monoclonal antibody of tumor necrosis factor and its preparation

A monoclonal antibody, necrosis factor technology, applied in the field of biomedicine, can solve problems such as easy to cause immune response in the human body

Active Publication Date: 2004-11-10
FOURTH MILITARY MEDICAL UNIVERSITY
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when these murine mAbs are used in humans, due to their immunogenicity, they are prone to induce immune responses in humans, which can cause clearance of antibodies and immune complex-mediated hypersensitivity reactions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Variable region gene of high neutralization activity monoclonal antibody of tumor necrosis factor and its preparation
  • Variable region gene of high neutralization activity monoclonal antibody of tumor necrosis factor and its preparation
  • Variable region gene of high neutralization activity monoclonal antibody of tumor necrosis factor and its preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0060] The present invention will be further described in detail below in conjunction with the accompanying drawings.

[0061] The complete nucleotide sequence of the variable region gene, the nucleotide sequence of its functional fragments (such as CDR, etc.), and the amino acid sequence of the antibody variable region are the basis for the construction of chimeric antibodies and humanized antibodies. Therefore, the applicant immunized BALB / c mice with recombinant human TNF, and prepared a group of mouse anti-human TNF monoclonal antibodies. And use monoclonal antibody to block the experiment of TNF-induced apoptosis of L929 cells, monoclonal antibody to block the experiment of TNF-induced ICAM-1 expression in ECV304 cells, and monoclonal antibody to block the experiment of TNF-induced NF-κB nuclear translocation, etc., screened out D2 hybridoma cell line with high neutralization activity. The strain of hybridoma cells has the ability to stably secrete antibodies.

[0062] ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a process for preparing variable zone genes of high neutralization activity tumor necrosis factor (TNF) resistant monoclonal antibody (D2 mAb) comprising, using recombinated human TNF immune BALB / c mouse to prepare a plurality of mouse Anti-TNF monoclonal antibody, carrying out extracorporal blocking-up experiment to screen anti-TNF monoclonal antibody with high neutralization activity. By cloning the monoclonal antibody light chain and heavy chain variable zone genes, the monoclonal antibody light chain and heavy chain variable zone gene sequence and amino acid sequence can be obtained, and the unicity of the gene sequence and protein sequence can be confirmed.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a monoclonal antibody gene, in particular to a preparation method of a variable region gene of a high neutralizing activity anti-tumor necrosis factor monoclonal antibody (D2 mAb). Background technique [0002] Tumor necrosis factor (TNF) is a homotrimeric soluble protein composed of three subunits with a molecular weight of 17kDa, mainly produced by monocytes and macrophages. A membrane-bound form of TNF with a molecular weight of 26 kDa is also present in vivo. TNF induces the expression of adhesion molecules such as intercellular adhesion molecule-1 (ICAM-1) on the surface of vascular endothelial cells, increases the adhesion of neutrophils and lymphocytes, and stimulates the secretion of macrophages, neutrophils and vascular endothelial cells A variety of cytokines and mediators cause inflammatory responses, resulting in damage to tissues such as bone and cartilage. At pre...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07H21/04C07K14/525C07K16/18C12P19/34C12P21/08
Inventor 金伯泉刘雪松杨琨朱参胜
Owner FOURTH MILITARY MEDICAL UNIVERSITY