Method for separation and purification of lecithin and cephalin

A technology for separation and purification of lecithin, which is applied in the field of separation and purification of lecithin and cephalin, which can solve the problems of small product processing capacity, high production cost, and large solvent consumption, and achieve large sample load, less solvent consumption, and reduced The effect of production costs

Inactive Publication Date: 2004-11-17
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the current high-purity lecithin and cephalin are mostly prepared by high-performance liquid chromatography (HPLC) eluting mode, the product processing capacity is small, and the solvent consumption is large, so the production cost is high and the price is expensive, thereby limiting its application.
In order to reduce the cost, many scholars have conducted research. For example, Kim et al. attempted to separate the egg yolk phospholipids from the perspective of simplifying the mobile phase by using pure methanol as the mobile phase, and achieved certain results, but they could not solve the problem fundamentally. Drawbacks Inherent in Wash Chromatography

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: The pump used for displacement chromatography separation is Shimadzu LC-6A, the chromatographic column is a 150mm×4.6mm self-packed silica gel column (3-5um), the volume of the injection loop is 0.5mL, and the mobile phase is dichloromethane-methanol (95:5, V / V), the displacing agent was 300 mM ethanolamine dissolved in the mobile phase solvent, and the flow rate was 0.2 mL / min. Weigh 100 mg of soybean mixed phospholipids, dissolve in 0.5 mL of dichloromethane-methanol (95:5, V / V) and inject. The eluate was collected every 0.2 mL. The purity and yield of the obtained lecithin were 90.6% and 76.5%, and the purity and yield of cephalin were 84.5% and 97.1%, respectively.

Embodiment 2

[0020] Example 2: The pump used for displacement chromatography separation is Shimadzu LC-6A, the chromatographic column is a 150mm×4.6mm self-packed silica gel column (5-10um), the volume of the injection loop is 0.5mL, and the mobile phase is dichloromethane-methanol (85:15, V / V), the displacing agent was 200 mM ethanolamine dissolved in the mobile phase solvent, and the flow rate was 0.2 mL / min. Weigh 100 mg of soybean mixed phospholipids, dissolve in 0.5 mL of dichloromethane-methanol (85:15, V / V) and inject. The eluate was collected every 0.2 mL. The purity and yield of the obtained lecithin were 88.8% and 80.2%, respectively, and the purity and yield of cephalin were 86.7% and 91.5%, respectively.

Embodiment 3

[0021] Embodiment three: The pump used for displacement chromatography separation is Shimadzu LC-6A, the chromatographic column is a 250mm × 4.6mm self-packed silica gel column (3-5um), the injection loop volume is 1mL, and the mobile phase is dichloromethane-methanol ( 95:5, V / V), the displacing agent was 100 mM ethanolamine dissolved in the mobile phase solvent, and the flow rate was 0.3 mL / min. Weigh 200 mg of soybean mixed phospholipids, dissolve in 1 mL of dichloromethane-methanol (95:5, V / V) and inject. The eluate was collected every 0.1 mL. The purity and yield of the obtained lecithin were 95.1% and 90.9% respectively, and the purity and yield of cephalin were 94.4% and 78.2% respectively.

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Abstract

The invention relates to a segregation and purification technology, in particular a method for separation and purification of lecithin and cephalin, wherein the mixture with main constituents of lecithine and cephalin obtained by pre-processing natural phosphatide is used as the raw material, silica gel is used as the fixed phase, methylene chloride - methanol is used as the mobile phase, aminoethyl alcohol is used as displacer for replaced chromatographic separation.

Description

technical field [0001] The invention relates to separation and purification technology, and relates to a method for separation and purification of lecithin and cephalin. Background technique [0002] Displacement chromatography was first proposed by Tswett in 1906, when he discovered that there was sample displacement in overloaded elution chromatography, but it was not until 1943 that Tiselius clearly defined chromatographic separation as frontier, elution and displacement. Displacement chromatography is a nonlinear chromatographic mode. Usually, one operation cycle includes four steps of equilibration, sample loading, displacement and regeneration. The force between the substance selected as the displacing agent and the stationary phase should be stronger than that between any component of the separated sample and the stationary phase. The separated sample is driven by the mobile phase with the displacing agent, and the components of the sample are fixed according to the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07F9/10C07K1/16C07K2/00
Inventor 达世禄冯钰锜张维农
Owner WUHAN UNIV
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