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Method for synchronous quick detecting multiple immune indexes by enzyme linked immunosorbent assay ELISA

An enzyme-linked immunosorbent absorption and rapid assay technology, which is applied in the field of medical microbiology and medical immunology detection, can solve the problems of long detection time, high detection cost, time-consuming and laborious, etc., and achieve fast detection speed, cost reduction, and low detection cost. Effect

Inactive Publication Date: 2004-12-15
苏冬梅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] The existing sandwich method and competition method can only detect one pathogen at a time. If multiple pathogens are to be detected, multiple preparations are required to test the antigen or antibody separately. The steps are cumbersome, the detection time is long, and time-consuming and laborious. high cost

Method used

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Examples

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specific Embodiment approach 1

[0053] Enzyme-linked immunosorbent assay (ELISA) method (sandwich method) for synchronous rapid determination of rotavirus and adenovirus pathogens, comprising the following sequential steps: preparation of test samples and control groups:

[0054] (1) Preparation of test samples:

[0055] (1) Coat the rotavirus-specific antibody and the adenovirus-specific antibody in the same polystyrene enzyme-linked plate well, and dry in the shade at 4°C;

[0056] (2) Extract the patient's specimen and add it to the well of the enzyme-linked plate for the test sample;

[0057] (3) Add the rotavirus antibody labeled with HRP (horseradish peroxidase) and the adenovirus antibody labeled with alkaline phosphatase into the wells of the enzyme-linked plate of the detection sample, wash the plate after sufficient reaction.

[0058] (2) Preparation of the control group

[0059] (1) Coat the rotavirus-specific antibody and the adenovirus-specific antibody in the same polystyrene enzyme-linked pl...

specific Embodiment approach 2

[0064] Enzyme-linked immunosorbent assay (ELISA) is a method (competition method) for the simultaneous rapid determination of rotavirus and adenovirus pathogens, comprising the following sequential steps:

[0065] Preparation of test samples and control groups:

[0066] (1) Preparation of test samples:

[0067] (1) Coat the rotavirus-specific antibody and the adenovirus-specific antibody in the same polystyrene enzyme-linked plate well, and dry in the shade at 4°C;

[0068] (2) Extract the patient's specimen and add it to the well of the enzyme-linked plate of the detection sample; at the same time

[0069] (3) Add HRP (horseradish peroxidase)-labeled rotavirus and alkaline phosphatase-labeled adenovirus antigens to the wells of the enzyme-linked plate of the test sample at the same time, wash the plate after sufficient reaction;

[0070] (2) Preparation of the control group

[0071] (1) Coat the rotavirus-specific antibody and the adenovirus-specific antibody in the same p...

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Abstract

The enzyme linked immunosorbent assay (ELISA) process for synchronously detecting several immune indexes quickly includes the following steps: coating two or more specific antibodies in the same enzyme linked plate holes and air drying at 4 deg.c after adsorption; setting extracted patient sample in the enzyme linked plate holes; adding enzyme labeled known antigens or antibodies in the number and sort corresponding the specific antibodies to enzyme linked plate holes before the plate is washed; adding primers corresponding different enzymes successively, color developing, and comparing with contrast for detection until finishing the detection. The present invention has the fast detection speed, saving in time and material and low in detection cost, and is suitable for epidemic disease survey.

Description

technical field [0001] The invention relates to medical microbiology and medical immunology detection technology, in particular to a method for measuring immune indexes by enzyme-linked immunosorbent assay (ELISA), more specifically, to a method for synchronously testing the immune index by enzyme-linked immunosorbent assay (ELISA). Method for rapid determination of multiple immune indexes Background technique [0002] Enzyme-linked immunosorbent assay (ELISA) is an important technology in clinical and experimental research at home and abroad, involving many sciences such as medicine, biology, genetics, and immunity. Known antigens or antibodies detect unknown antibodies or antigens, especially in the diagnosis of diseases and the observation of curative effects and preventive medicine, such as medically used to diagnose and differentially diagnose hepatitis viruses (A, B, C, D, E, Hepatitis G virus), AIDS diagnosis and other bacterial, chlamydia, protozoa infection diagnos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/547
Inventor 苏冬梅
Owner 苏冬梅