Flavor-active peptides
A fragrance and active technology, applied in the field of special peptides, can solve the problem of lack of chocolate aroma, intensity, rich quality, etc.
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[0111] 80kg piles of high-quality West African Amelonado cocoa beans are fermented in banana leaves at the Abengoorou Idefor farm in Ivory Coast. Samples were taken at 1 day intervals during the 7 day fermentation cycle, sun dried, hand peeled and shipped to NRC / Lausanne. Cocoa beans are stored at -20°C.
[0112] Synthetic peptides were obtained from Bachem. HPLC grade acetonitrile, methanol and water were from Merck. Trifluoroacetic acid, triethylamine and fluororoscamine were from Fluka.
[0113] Preparation of soluble peptide extract
[0114] The dried cocoa beans were passed through a bean crusher (Brook Crampon) followed by a winnowing machine (John Gordon) to remove the shells. Store the soy meat in a brown bottle at -20°C. The cocoa nibs (20-30 g) are ground for a few seconds in a common mill (IKA, M-20). The soymeal was passed through a 0.8-mm sieve and stored at 4°C (NRC references 14C181-0 to 14C18-7).
[0115] Cocoa nib powder (1 g) was suspended in 10 ml of ...
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