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Pathogenic bacteria immune detection chip and preparation method thereof

An immunological detection and pathogenic technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of time-consuming and labor-intensive, false positives, etc., and achieve the effect of reliable results and time-saving and labor-saving results.

Inactive Publication Date: 2005-04-06
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also some reports on other detection methods, such as the identification of different microorganisms by detecting the specific 16S rRNA of different microorganisms. These methods have technical defects such as high false positives, time-consuming and laborious, etc.

Method used

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  • Pathogenic bacteria immune detection chip and preparation method thereof

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Embodiment 1

[0011] 1.. Glass handling

[0012] 1).Slide cleaning

[0013] Clean the glass slides with water, put them into a solution consisting of 1 / 3 hydrogen peroxide and 2 / 3 concentrated sulfuric acid, and soak for 0.5-3.0 hours. Then rinse with deionized water or distilled water for 2-6 times, and then boil with deionized water or distilled water for 2-30 minutes; dry under nitrogen flow, and store in a dry place for future use.

[0014] (1). Aminosilanization of the surface of the glass slide. The cleaned glass slide is immersed in a 95% acetone / water solution containing 0.1-10.0% aminopropyltrimethoxysilane (3-aminopropyltriethoxysilane) for 3-30 minutes, and then taken out Finally, the slides were washed 2-6 times with acetone, 1-5 minutes each time, then rinsed 1-5 times with deionized distilled water, 1-5 minutes each time, and finally dried at 60-180 ° C, placed in a dry save.

[0015] (2). Aldehyde modification on the glass slide surface

[0016] Put the silanized glass sl...

Embodiment 2

[0026] The product described in the present invention is a pathogenic bacteria immune detection chip, comprising: a carrier 1 on which bacterial antibodies 2 different from each other are immobilized by chemical groups containing amino groups or aldehyde groups, and the same The bacterial antibodies are arrayed on the carrier.

Embodiment 3

[0028] The method of the present invention is a preparation method for manufacturing pathogenic bacteria immune detection chip, first take the glass slide and clean it, and then immerse the cleaned glass slide in the chip containing 0.1-10.0% aminopropyl trimethoxy 95% acetone / water solution of base silane for 3-30 minutes. After taking it out, wash the glass slide 2-6 times with acetone, 1-5 minutes each time, and then rinse 1-5 times with deionized distilled water, 1 time each time. -5 minutes, then dry at 60-180°C to silanize the surface of the slide, then modify the amino group or aldehyde group on the surface of the slide, and finally, set the pathogenic bacteria antibodies on the slide in an array on the chip, and store it in a humid environment at 0°C-55°C for 0.1-72 hours, take it out, wash it with a buffer solution with pH 4.0-10, ion concentration 0.00-0.75m / L, and wash it 1-5 times. The tablet is cleaned with water, put into the mixed liquid composed of hydrogen per...

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Abstract

This invention product is a pathogenic bacterial immune test chip and its process method is the following: first to put cleaned slide into solvent with 0.1 to 10 percent of amidol propyl trimethyl silane and 95 percent of acetone and water for 3 to 30 minutes; to get slide out and wash it for two to six time with one to five minutes each; then to wash it with ion distilled water for one to five times with one to five minutes each; then to dry it in temperature of 60 to 180 degrees and make the surface of slide silanizated; then to modify the amidol or propyl of the slide surface; final to arrange the pathogenic bacterial antibody on the slide and store it on wet environment for 0.1 to 72 hours.

Description

technical field [0001] The invention relates to a pathogenic bacteria immune detection chip for detecting pathogenic bacteria in feces, soil, water, food, fermented materials, etc., as well as water and environment, and a preparation method thereof. Background technique [0002] Although there are many types of pathogenic bacteria, each genera has its own characteristics in terms of morphology and structure. Generally, it can be identified by different staining methods or by microscopy. At present, there is a biochemical culture method for bacterial testing. The advantage of this method is that the results of the detected bacteria are reliable, but the disadvantage is that the detection takes a long time. During the bacterial culture process, some bacteria may be inhibited and affect the reliability of the detection results. Needle-labeled immunization method; the detection result is reliable, but the disadvantage is that the probe label is expensiv...

Claims

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Application Information

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IPC IPC(8): G01N33/531G01N33/569
Inventor 唐祖明陆祖宏郁颖蕾史智扬
Owner SOUTHEAST UNIV
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