Pathogenic bacteria immune detection chip and preparation method thereof

An immunological detection and pathogenic technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of time-consuming and labor-intensive, false positives, etc., and achieve the effect of reliable results and time-saving and labor-saving results.

Inactive Publication Date: 2005-04-06
SOUTHEAST UNIV
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also some reports on other detection methods, such as the identification of different microorganisms by detecting the specific 1

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pathogenic bacteria immune detection chip and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0010] Example 1

[0011] 1. Glass slide treatment

[0012] 1). Slide cleaning

[0013] Take the slides, wash them with water, put them in a solution composed of 1 / 3 hydrogen peroxide and 2 / 3 concentrated sulfuric acid, and soak them for 0.5-3.0 hours. Then rinse with deionized water or distilled water for 2-6 times, then boil with deionized water or distilled water for 2-30 minutes; dry under nitrogen flow, and store in a dry place for future use.

[0014] (1) Aminosilanization on the surface of the glass slide The cleaned glass slide was immersed in a solution of 95% acetone / water containing 0.1-10.0% aminopropyltriethoxysilane for 3-30 minutes, and then taken out After that, the slides were washed 2-6 times with acetone for 1-5 minutes each time, and then washed with deionized distilled water for 1-5 times for 1-5 minutes each time, and finally dried at 60-180°C and left to dry. save at.

[0015] (2). Aldehyde group modification on the surface of glass slide

[0016] P...

Example Embodiment

[0025] Example 2

[0026] The product of the present invention is a pathogenic bacteria immunodetection chip, comprising: a carrier 1, on which different bacterial antibodies 2 are immobilized by chemical groups containing amino or aldehyde groups arranged on the carrier, and the same The bacterial antibodies are arrayed on the carrier.

Example Embodiment

[0027] Example 3

[0028] The method of the invention is a preparation method for manufacturing a pathogenic bacteria immunodetection chip. First, a glass slide is taken and cleaned, and then the cleaned glass slide is immersed in 0.1-10.0% aminopropyltrimethoxy The solution of 95% acetone / water of base silane for 3-30 minutes, after taking out, the slides were washed 2-6 times with acetone, 1-5 minutes each time, and then rinsed with deionized distilled water 1-5 times, 1 time each time -5 minutes, then dried at 60-180 °C to silanize the surface of the glass slide, then, modify the amino group or aldehyde group on the surface of the glass slide, and finally, set the pathogenic bacterial antibody on the glass slide according to the array. On the slide, put it in a humid environment of 0℃-55℃ for 0.1-72 hours, take it out, wash it 1-5 times with buffer solution with pH 4.0-10, ion concentration 0.00-0.75m / L, the above glass slides The cleaning of the tablet is to clean it with...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

This invention product is a pathogenic bacterial immune test chip and its process method is the following: first to put cleaned slide into solvent with 0.1 to 10 percent of amidol propyl trimethyl silane and 95 percent of acetone and water for 3 to 30 minutes; to get slide out and wash it for two to six time with one to five minutes each; then to wash it with ion distilled water for one to five times with one to five minutes each; then to dry it in temperature of 60 to 180 degrees and make the surface of slide silanizated; then to modify the amidol or propyl of the slide surface; final to arrange the pathogenic bacterial antibody on the slide and store it on wet environment for 0.1 to 72 hours.

Description

technical field [0001] The invention relates to a pathogenic bacteria immune detection chip for detecting pathogenic bacteria in feces, soil, water, food, fermented materials, etc., as well as water and environment, and a preparation method thereof. Background technique [0002] Although there are many types of pathogenic bacteria, each genera has its own characteristics in terms of morphology and structure. Generally, it can be identified by different staining methods or by microscopy. At present, there is a biochemical culture method for bacterial testing. The advantage of this method is that the results of the detected bacteria are reliable, but the disadvantage is that the detection takes a long time. During the bacterial culture process, some bacteria may be inhibited and affect the reliability of the detection results. Needle-labeled immunization method; the detection result is reliable, but the disadvantage is that the probe label is expensiv...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/531G01N33/569
Inventor 唐祖明陆祖宏郁颖蕾史智扬
Owner SOUTHEAST UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap