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Preparation method of compound egg yolk antibody medicinal preparation for preventing and treating piglet virus epidemic disease

A yolk antibody and viral technology, applied in the direction of antiviral agents, antibodies, etc., can solve the problems of high cost, complicated preparation process, etc., and achieve the effects of low cost, quick effect, and simple use method.

Inactive Publication Date: 2005-08-10
SHANGHAI JIAO TONG UNIV
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AI Technical Summary

Problems solved by technology

However, this patent needs to purify the egg yolk antibody before use. The preparation process is cumbersome and the cost is high. A single antibody is ineffective against diarrhea caused by other pathogens. The purified anti-rotavirus egg yolk antibody is only used in infants and young children.

Method used

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Embodiment Construction

[0009] Raise clean-grade laying hens. In the first round, they will be immunized with transmissible gastroenteritis and porcine epidemic diarrhea vaccines, and then immunized with rotavirus vaccines 5 days later. The immunization dose is 1 head. The second round of immunization was carried out at an interval of 14 days, and the immunization dose was 2 doses. The third round of immunization was carried out 14 days after immunization, and the immunization dose was 4 heads. 10 days after the end of the third round of immunization, after the titer of egg yolk antibody was determined (latex agglutination test titer reached 1:40 or more), the immunized eggs were collected, and the egg yolk was aseptically obtained, diluted with 0.01M sterile phosphate buffer solution, 0.5 % hydroxypropylmethylcellulose phthalate, the filtrate was spray-dried, and the powder was packaged and preserved. The yield of immunoglobulin is more than 90%.

[0010] For the diarrhea caused by this disease in...

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Abstract

A compound yolk antibody preparation for preventing the viral diseases of piglet, including viral diarrhea of piglet is prepared through providing the vaccines of infectious gastroenteritis, epidemic diarrhea and rotavirus, immunizing with them sequentially by three turns, collecting immunized eggs, taking yolk, diluting with aseptic buffer liquid of phosphate, treating by hydroxypropylmethyl cellulose phthalate, filtering, and spraying drying.

Description

technical field [0001] The invention relates to a preparation method of an antibody preparation in the field of biotechnology, in particular to a preparation method of a composite egg yolk antibody preparation against viral disease of piglets. Background technique [0002] The pathogens of enteroviral infectious diseases mainly caused by diarrhea in piglets mainly include transmissible gastroenteritis virus, porcine epidemic diarrhea virus and rotavirus, which are characterized by acute onset, wide prevalence and high mortality, which seriously hinders the pig industry development of. In production, the vaccines prepared by these viruses are mainly used to immunize pregnant sows, and protect piglets from colostrum, but the effect is poor. There is no specific treatment drug for this disease at present, and the prognosis is poor after the onset. [0003] Since Yokoyama et al. found in 1992 that yolk antibodies can treat E. coli diarrhea in piglets, yolk antibodies have been...

Claims

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Application Information

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IPC IPC(8): A61K39/395A61P31/12
Inventor 徐建雄刘永德叶陈梁
Owner SHANGHAI JIAO TONG UNIV
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