Method for establishing symboitic relationship for arbuscular nycorrhizal fungi and tomato hairy root

A symbiotic relationship and hairy root technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems that sterile conditions cannot be obtained and AM fungi cannot be purely cultivated

Active Publication Date: 2005-10-19
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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AI Technical Summary

Problems solved by technology

However, until now, AM fungi cannot be cultured purely and must establish a symbiotic relationship with the host plant to complete the life cycle
Because of this, the current breeding of AM fungi in our country is mainly carried out by cultivating host plants in substrates. These substrates mainly include soil, river sand, vermiculite, glass beads, etc. Under these culture conditions, sterile conditions cannot be obtained.
Research on the biological characteristics of arbuscular mycorrhizal fungi and the interaction with soil microorganisms needs to be carried out under sterile conditions without matrix interference, so the previous AM fungal culture method is difficult to apply to such research

Method used

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  • Method for establishing symboitic relationship for arbuscular nycorrhizal fungi and tomato hairy root
  • Method for establishing symboitic relationship for arbuscular nycorrhizal fungi and tomato hairy root

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Embodiment 1

[0018] 1. Induction of tomato hairy roots:

[0019] Take 10 ruby ​​and Fengshun tomato seeds each, put them in water for 12-24 hours, sterilize with 10% sodium hypochlorite for 15-20 minutes, wash with sterile water 5 times, and sow them on 2% sucrose agar medium ; After the culture dish was placed in the dark at 25°C for 7 to 10 days, the seedlings were cut into three parts: hypocotyl, mesocotyl and epicotyl on the ultra-clean workbench. 7 ~10 8 Cfu / ml Agrobacterium rhizogenes (Agrobacterium rhizogenes) R1000 bacterial suspension soaked for 20-30 minutes, washed 5 times with sterile water after taking it out, then wiped dry the water on the surface of plant tissue with sterile filter paper, inoculated to 2% cultured on sucrose agar medium at 25°C under dark conditions; during the cultivation period, the formation of hairy roots was often observed, and hairy roots began to appear on the surface of plant tissues after about 7 to 15 days; when the hairy roots grew to 2 to 5 cm ...

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Abstract

The present invention relates to the method of establishing symbiotic relationship between arbuscular nycorrhizal fungus and tomato hairy root. Germinated tomato seedling is first soaked with agrolacterium suspension and inoculated to sucrose-agar culture medium for culture to induce hairy root; the tomato hairy root is then transferred to M culture medium and AM fungi spore is inoculated for dark culture at 25 deg.c; and the germinated hypha invades into root system to establish the symbiotic relationship between arbuscular nycorrhizal fungus and tomato hairy root. The present invention induces tomato hairy root and establishes the symbiotic relationship between AM fungus and tomato hairy root on bacteria-free culture medium. The system of the present invention may be used in the amplification of AM fungus to obtain bacteria-free AM fungus material and in the relevant research of AM fungus under bacteria-free condition.

Description

[Affiliated technical field] [0001] The invention relates to a method for establishing a symbiotic relationship between arbuscular mycorrhizal fungi and tomato hairy roots. [Background technique] [0002] Arbuscular mycorrhizal fungi (Arbuscular Mycorrhizal Fungi, AM fungi) are a class of soil fungi that can establish a symbiotic relationship with 80% of plant species. It has long been recognized that AM fungi can significantly promote the absorption of mineral nutrients by plant roots and enhance plant resistance to various soil-borne diseases, thereby improving plant growth. Therefore, scientists from various countries agree that AM fungi have broad application prospects in most crops and horticultural crops, and call them "biological fertilizers" and "biological pesticides" (Scientia Horticulturae, 1997, 68: 1-24). At present, the research on AM fungi is becoming more and more extensive and in-depth. Many studies need to be carried out under sterile conditions, or steril...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00A01G7/06A01H4/00
Inventor 朱红惠姚青羊宋贞龙良鲲
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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