Method for establishing symboitic relationship for arbuscular nycorrhizal fungi and tomato hairy root

Abstract

The present invention relates to the method of establishing symbiotic relationship between arbuscular nycorrhizal fungus and tomato hairy root. Germinated tomato seedling is first soaked with agrolacterium suspension and inoculated to sucrose-agar culture medium for culture to induce hairy root; the tomato hairy root is then transferred to M culture medium and AM fungi spore is inoculated for dark culture at 25 deg.c; and the germinated hypha invades into root system to establish the symbiotic relationship between arbuscular nycorrhizal fungus and tomato hairy root. The present invention induces tomato hairy root and establishes the symbiotic relationship between AM fungus and tomato hairy root on bacteria-free culture medium. The system of the present invention may be used in the amplification of AM fungus to obtain bacteria-free AM fungus material and in the relevant research of AM fungus under bacteria-free condition.

Description

[Affiliated technical field] [0001] The invention relates to a method for establishing a symbiotic relationship between arbuscular mycorrhizal fungi and tomato hairy roots. [Background technique] [0002] Arbuscular mycorrhizal fungi (Arbuscular Mycorrhizal Fungi, AM fungi) are a class of soil fungi that can establish a symbiotic relationship with 80% of plant species. It has long been recognized that AM fungi can significantly promote the absorption of mineral nutrients by plant roots and enhance plant resistance to various soil-borne diseases, thereby improving plant growth. Therefore, scientists from various countries agree that AM fungi have broad application prospects in most crops and horticultural crops, and call them "biological fertilizers" and "biological pesticides" (Scientia Horticulturae, 1997, 68: 1-24). At present, the research on AM fungi is becoming more and more extensive and in-depth. Many studies need to be carried out under sterile conditions, or steril...

AI Technical Summary

Problems solved by technology

However, until now, AM fungi cannot be cultured purely and must establish a symbiotic relationship with the host plant to complete the life cycle

Because of this, the current breeding of AM fungi in our country is mainly carried out by cultivating host plants in substrates. These substrates mainly include soil, river sand, vermiculite, glass beads, etc. Under these culture conditions, sterile conditions cannot be obtained.

Research on the biological characteristics of arbuscular mycorrhizal fungi and the interaction with soil microorganisms needs to be carried out under sterile conditions without matrix interference, so the previous AM fungal culture method is difficult to apply to such research

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