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Efficient parts of hydrangea paniculata, their preparing method and compositions and use thereof

A technology of effective parts, cone hydrangea, applied in the directions of medical preparations containing active ingredients, pharmaceutical combinations, preparation of sugar derivatives, etc.

Active Publication Date: 2005-11-02
INST OF MATERIA MEDICA CHINESE ACAD OF MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pane hydrangea is rich in natural resources, and no toxic reports have been seen

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1 kg of roots or stems of Hydrangea paniculata were refluxed three times with 95% ethanol for 2 hours each time, concentrated under reduced pressure at 60° C. to obtain extracts. This extract is dissolved in ethanol: tetrahydrofuran: a small amount of water (8:8:1), and the supernatant is mixed with 100-200 mesh 250g silica gel as a sample, dried in a water bath at 60°C, passed through a 100-200 mesh 100g silica gel column, respectively Elute with 3500ml of petroleum ether: acetone (1:1), 2000ml of acetone, and ethanol, collect the acetone eluate, concentrate under reduced pressure at 60°C to obtain each fraction respectively. The acetone fraction (crude fraction) was dissolved with ethanol: tetrahydrofuran: water (7:8:3), passed through an ODS column, and eluted with 10-15% ethanol mobile phase to obtain a hydrangea extract containing more than 50% inosin.

Embodiment 2

[0049] 1 kg of roots or stems of Hydrangea paniculata were refluxed three times with 95% ethanol for 2 hours each time, concentrated under reduced pressure at 60° C. to obtain extracts. This extract is dissolved in ethanol: tetrahydrofuran: a small amount of water (8:8:1), and the supernatant is mixed with 100-200 mesh 250g silica gel as a sample, dried in a water bath at 60°C, passed through a 100-200 mesh 100g silica gel column, respectively Elute with different concentrations of 3500ml petroleum ether: acetone (1:1), 2000ml acetone, ethanol, collect the acetone eluate, concentrate the acetone eluate at 60°C under reduced pressure to obtain the acetone fraction (crude fraction). The acetone part is subjected to macroporous resin column chromatography and eluted with 10-40% ethanol to obtain hydrangea paniculata extract containing more than 50% inosin.

Embodiment 3

[0051] 1 kg of roots or stems of Hydrangea paniculata were refluxed three times with 95% ethanol for 2 hours each time, concentrated under reduced pressure at 60° C. to obtain extracts. This extract ethanol: tetrahydrofuran: a small amount of water (8: 8: 1) dissolves, gets the supernatant and 100-200 order 250g silica gel to mix sample, water bath 60 ℃ of oven dry, carry out silica gel column chromatography (loading amount and silica gel ratio), with CH 3 Cl:MeOH:H 2 O (8:2:0.1) mobile phase was eluted to obtain the Hydrangea paniculata extract containing more than 50% inosin.

[0052] Pharmacological experiment

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PUM

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Abstract

The invention discloses a significant position extracted from hydrangea paniculata sieb and the method for producing it, medical compounds containing such position, and the usage of it, especially in producing drugs for preventing and / or curing kidney incompetence, high blood pressure and diabetes, kidney disease.

Description

[0001] technical field [0002] The present invention relates to the effective part extracted from Hydrangea paniculata, the preparation method of the effective part, the pharmaceutical composition containing the effective part, and the use of the effective part and its pharmaceutical composition as medicine, especially in the preparation of preventive and / or the application of drugs in the treatment of renal insufficiency, hypertension and diabetic nephropathy. Background technique [0003] Chronic kidney disease (including various types of chronic nephritis, diabetic nephropathy and hypertensive renal damage, etc.) is the most common chronic refractory disease. The annual incidence rate of chronic renal failure (CRF) in the natural population is 98-198 / 1 million population, and the incidence rate in developed countries such as the United States and Japan is as high as 8-10 million / 1 million population, showing an exponential upward trend in the past ten years. The exact ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08
Inventor 张东明陈晓光杨敬芝李燕郑旭光
Owner INST OF MATERIA MEDICA CHINESE ACAD OF MEDICAL SCI
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