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Method for preparing hirsutella polysporaZK10 of parasitic nematode and use thereof

A technology of trichomes and fungi, applied in fungi and other directions, can solve problems such as drug resistance, side effects of chemical nematicides, and harm to human health.

Inactive Publication Date: 2005-11-02
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the advancement of science and technology, it has been found that many chemical nematicides have side effects, which not only produce drug resistance, but also cause environmental pollution and endanger human health. Many of them have been banned or will be banned one after another.

Method used

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  • Method for preparing hirsutella polysporaZK10 of parasitic nematode and use thereof
  • Method for preparing hirsutella polysporaZK10 of parasitic nematode and use thereof
  • Method for preparing hirsutella polysporaZK10 of parasitic nematode and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Hirsutella polyspora ZK10 cells were inoculated on the slant of test tube agar medium, and the medium formula was: 1.5% yeast extract, 1% peptone; 3% glucose; 2% agar; the pH was natural. The ZK10 mycelium was inoculated on the culture medium and cultured at 22°C for 23 days to obtain test tube species.

[0040] Inoculate the test tube species on a petri dish with a diameter of 9 cm. The medium formula is the same as the above test tube agar medium. After inoculating ZK10, seal it with a parafilm and culture it at 22°C for 25-30 days. It can be used when spores are produced.

Embodiment 2

[0042] It is basically the same as Example 1, except that the formula of the liquid medium is: 1% yeast extract, 2% peptone; 2% glucose; 2% agar.

Embodiment 3

[0044] It is basically the same as Example 1, except that the formula of the liquid medium is: 2% yeast extract, 0.5% peptone; 4% glucose; 2% agar.

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Abstract

The invention relating to a method for preparation and utility of hirsutella polyspora fungi straying wireworm belongs to biotic pesticide technical sphere. The production strain is: Hirsutella polyspora ZK10, prepared in conventional method, and the formulation of culture medium is: yeast extract of 1 to 2 percent, peptone of 0.2 to 2 percent; dextroglucose of 2 to 4 percent; agar of 2 percent; and PH spontaneous. Prepare the lawn plate for testing with the said culture medium using the culture dish with a diameter of 9 cm in the conventional method, and it can be used when spore is produced largely. The strain carrys its point of wireworm prevention by straying wireworm, and the product has a high straying rate to pine beam wireworm and Panagrellus redivivus wireworm, having a good latent prospect for application.

Description

Technical field: [0001] The invention relates to a preparation method and application of a polyspora trichospora fungus of parasitic nematodes, belonging to the technical field of biological pesticides. Background technique: [0002] Plant nematodes are a common plant disease worldwide. There are more than 70 known species of root-knot nematodes alone, which harm more than 3,000 plants and cause huge losses every year. According to the statistics of the Food and Agriculture Organization of the United Nations, the global losses caused by nematodes are as high as 100 billion U.S. dollars every year. In 2001, the losses caused by nematode damage to crops in my country were as high as 23.3 billion yuan. Pine xylophilus is known as smokeless forest fires, which have seriously occurred in some areas such as Jiangsu, Zhejiang, Guangdong, Shandong, Anhui, Hubei, Shanghai, Taiwan, Hong Kong, etc., and have a tendency to spread to the whole country. What's more serious is that root-k...

Claims

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Application Information

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IPC IPC(8): C12N1/14
Inventor 张克勤李萱罗宏莫明和
Owner YUNNAN UNIV
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