Quick breeding method for inducing spheroidal embryo directly from tissue of anthurium

A technology of anthurium and spherical shape, which is applied in the field of rapid propagation of anthurium tissue to directly induce spherical embryo clusters, to achieve the effect of convenient material selection, large amount of rapid propagation seedlings, and low price

Inactive Publication Date: 2005-11-16
天津龙康泰生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Chinese patent 200310109713.3 discloses a method for rapid propagation of anthuria somatic embryo induction, but the rapid propagation method of anthuria tissue for one-step induction of globular embryo mass has not been reported

Method used

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  • Quick breeding method for inducing spheroidal embryo directly from tissue of anthurium
  • Quick breeding method for inducing spheroidal embryo directly from tissue of anthurium
  • Quick breeding method for inducing spheroidal embryo directly from tissue of anthurium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: Leaf, blade stalk end tissue culture forms spherical embryo group and seedling

[0035] Take the young leaves of mature plants or young plants (flower seedlings) of Anthurium (taken from the greenhouse of the flower sales office of Guolian Flower Base, Changchun City, Jilin Province), and sterilize as above (wash with tap water first, then rinse with distilled water for 3 times , then put in 75% ethanol for 1 minute, rinse twice with sterile water, soak with mercuric chloride or sodium hypochlorite for 5-10 minutes, take it out, and rinse with sterile water for 3 times, then it can be used as tissue culture material) , shredded under aseptic conditions as Figure 10 , put into the above medium: 1 / 2MS (basic medium) + BA (6-benzyl adenine) 0.5 mg / L + NAA (α-naphthalene acetic acid) 0.1 mg / L + KT (N 6 -furylmethyladenine) 0 mg / L+2,4-D (2,4-dichlorophenoxyacetic acid) 0 mg / L+3% sucrose. Cultivate under light, light for 7-15 hours a day, light intensity is 3...

Embodiment 2

[0036] Embodiment 2: Stem axis, stem tip, stem node tissue culture form spherical embryo group and seedling

[0037] Take Anthurium anthurium (taken from the greenhouse of the flower sales office of Guolian Flower Base, Changchun City, Jilin Province) as an adult plant or seedling, remove its leaves and get its stem axis, stem tip, and stem nodes, and sterilize as above (wash with tap water first, Rinse with distilled water for 3 times, then put in 75% ethanol for 1 minute, rinse with sterile water for 2 times, soak in mercuric chloride or sodium hypochlorite for 5-10 minutes, take it out, and rinse with sterile water for 3 times. Reserved as tissue culture material), it was minced under sterile conditions as Figure 13, put into the above medium: 1 / 2MS (basic medium) + BA (6-benzyl adenine) 1 mg / L + NAA (α-naphthalene acetic acid) 0.1 mg / L + KT (N 6 -furyl methyl adenine) 0.1 mg / L+2,4-D (2,4-dichlorophenoxyacetic acid) 0 mg / L+3% sucrose or glucose were put on the above-menti...

Embodiment 3

[0038] Embodiment 3: Petiole, aerial root tip tissue culture form spherical embryo group and seedling

[0039] Take the petiole, aerial root tip or petiole of the seedling of Anthurium anthurium (taken from potted flowers in Changchun Flower, Bird, Fish and Insect Wholesale Market), and sterilize as above (wash with tap water first, then rinse with distilled water for 3 times, then put into 75% ethanol for 1 minute, rinse twice with sterile water, soak in mercuric chloride or sodium hypochlorite for 5-10 minutes, take it out, and rinse with sterile water for 3 times, ready to be used as tissue culture material), in the absence of Under bacterial conditions, it was cut into 3 mm long segments such as Figure 16 , into the above medium: 1 / 2MS (basic medium) + BA (6-benzyl adenine) 1mg / L + 2,4-D (2,4-dichlorophenoxyacetic acid) 0.1mg / L + KT(N 6 -furylmethyladenine) 0mg / L+3% sucrose, cultivate under light, light 7-15 hours every day, light intensity is 25000 lux, change culture ...

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Abstract

A fast reproduction method for Anzu flower includes taking the tissue of Anzu flower, using it as explant, culturing it in culture medium while controlling the development of calli, using the calli to directly induce globular embryo cluster, and using the globular embryo cluster for fast reproduction.

Description

technical field [0001] The invention relates to the rapid propagation technology of flowers; in particular, a rapid propagation method for directly inducing globular embryo mass by anthurium tissue. The spherical embryo group is directly induced by the callus, and then the same medium is used to make the spherical embryo group grow 5-300 seedlings for rapid propagation. Its advantages are that the selection of rapid propagation materials is convenient, accurate, fast, and rapid propagation of seedlings Large, stable, and simple, avoiding the complicated technical procedures and processes of somatic embryo induction. The reproduction quantity of the plants can be used for industrialized production, the price is low, the operation is convenient, and the method is practical for enterprises. Background technique [0002] Anthurium (anthurium), also known as anthurium, anthurium, anthurium, etc., is mainly cultivated by hybridization of two species, andreanum and scherzerianum. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00
Inventor 季静王罡姚振孙文君王萍
Owner 天津龙康泰生物技术有限公司
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