Method for quantitative detecting water quality in oil field through sulfate reducting bacteria

Abstract

A method for quantitatively testing the water quality in oil field by use of sulfate reducing bacterium (SRB) includes taking 3-5 parallel samples in same dilution gradient, filling them in culture media, putting the MPN culture media on shaker, shaking at 38 deg.C and 180 rpm and respective counting in the fourth and the seventh days.

Description

Technical field:

[0001] The invention relates to the cultivation and counting of sulfate-reducing bacteria (SRB) in the water treatment process of conventional sewage, poly-containing sewage and surface technology in an oil field water system. Background technique:

[0002] At present, the counting of SRB bacteria in the oilfield system mainly follows the standards of China's oil and gas industry, "Bacterial Analysis Method for Oilfield Injection Water - Extinction Dilution Method" (Ministerial Standard), SY-TO532-93. The problem with the above-mentioned method is that the detection takes 14 days. Due to the long detection cycle, it cannot effectively and immediately guide the production practice. In the actual operation process, some strict anaerobic SRB bacteria cannot survive, resulting in SRB The counting result of bacteria is inaccurate and cannot truly reflect the actual production situation, and the detection cost is high at the same time. Invention content: [000...