Method for quantitative detecting water quality in oil field through sulfate reducting bacteria

A quantitative detection method, sulfate technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of long detection cycle, inability to guide production in real time, shorten counting time, etc.

Inactive Publication Date: 2005-11-16
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to solve the problems of the existing oil field water quality SRB bacteria detection, the detection cycle is long, the number of SRB bacteria in the water cannot be truly and comprehensively reflected, the production cannot be guided immediately, and the detection cost is high. A quantitative detection method for sulfate-reducing bacteria in water quality, the present invention has the characteristics of accurate counting, shortened counting time, true reflection of actual production conditions, and reduced detection costs

Method used

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specific Embodiment approach 1

[0004] Specific embodiment one: the method of this embodiment is realized through the following steps: 1, sampling: inject the water sample of oilfield into the anaerobic tube that contains high-purity nitrogen through sterilizing; Finish under the stage, sterilize with ultraviolet light for 20-40 minutes, add 1 mL of FeSO with a mass percentage concentration ratio of 6% to the culture medium of sterilized sulfate-reducing bacteria 4 ·(NH 4 ) 2 SO 4 Solution (final concentration is 6‰) standby; 3, "anaerobic MPN method", select the dilution factor of the water sample: usually two to three orders of magnitude greater than the empirical value, dilute with the multiple dilution method (MPN), and take the water sample 1mL was injected into the anaerobic tube for doubling dilution, and the final water sample was diluted ten times, and 1mL was sequentially sampled from this tube with a disposable syringe and injected into the next anaerobic tube for doubling dilution until the sel...

specific Embodiment approach 2

[0005] Specific embodiment two: the preparation method of the sulfate-reducing bacteria medium of the present embodiment is as follows: one, at first prepare medium by weight parts by following composition, K 2 HPO 4 : 0.2~0.8, NH 4 CI: 0.5-1.5, Na 2 SO 4 : 5~15, CaCI 2 2H 2 O: 0.01~0.6, MgSO 4 ·7H 2 O: 1.5 ~ 4, yeast extract: 4 ~ 8, sodium lactate solution with a mass percentage concentration ratio of 60%: 5 ~ 15, glucose: 0.5 ~ 1.5, vitamin C: 1 ~ 5, distilled water: 1000 ~ 2000, the above Mix the ingredients evenly, then adjust the pH value to 7.8 to make a culture medium, and then add an indicator resazurin solution of 0.2% of the total weight of the medium; 2. After boiling the above medium in a sterilized pot, add culture medium Based on 0.2-0.8% of the total weight of L-cysteine ​​salt, cover the pot, insert a medical needle through the middle hole of the pot, and inject high-purity nitrogen to drive oxygen for 20-30 minutes. The color of the indicator gradually ...

specific Embodiment approach 3

[0006] Specific embodiment three: the mass percent concentration ratio of this embodiment is 6% FeSO 4 ·(NH 4 ) 2 SO 4 The preparation method of the solution is as follows: first prepare anaerobic water, add one liter of deionized water to the sterilizer and boil it, then add 0.2-0.8g of L-cysteine ​​salt, cover the lid of the sterilizer, and remove it from the lid Insert a medical needle into the middle hole of the sterilizer, and inject high-purity nitrogen gas into the sterilizer to drive oxygen for 10-30 minutes. Prepare 10 mL of 6% FeSO 4 ·(NH 4 ) 2 SO 4 solution, the weighed FeSO 4 ·(NH 4 ) 2 SO 4 Put it into the anaerobic tube, insert another medical needle into the anaerobic tube, pass high-purity nitrogen gas, blow away the oxygen-containing air in the anaerobic tube, and use a 10mL medical syringe to suck 10mL of water from the sterilizing pot to inject For the anaerobic tube, sterilize the sealed anaerobic tube at 121°C for 15-30 minutes. Other methods a...

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Abstract

A method for quantitatively testing the water quality in oil field by use of sulfate reducing bacterium (SRB) includes taking 3-5 parallel samples in same dilution gradient, filling them in culture media, putting the MPN culture media on shaker, shaking at 38 deg.C and 180 rpm and respective counting in the fourth and the seventh days.

Description

Technical field: [0001] The invention relates to the cultivation and counting of sulfate-reducing bacteria (SRB) in the water treatment process of conventional sewage, poly-containing sewage and surface technology in an oil field water system. Background technique: [0002] At present, the counting of SRB bacteria in the oilfield system mainly follows the standards of China's oil and gas industry, "Bacterial Analysis Method for Oilfield Injection Water - Extinction Dilution Method" (Ministerial Standard), SY-TO532-93. The problem with the above-mentioned method is that the detection takes 14 days. Due to the long detection cycle, it cannot effectively and immediately guide the production practice. In the actual operation process, some strict anaerobic SRB bacteria cannot survive, resulting in SRB The counting result of bacteria is inaccurate and cannot truly reflect the actual production situation, and the detection cost is high at the same time. Invention content: [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06
Inventor 马放魏利陈忠喜
Owner HARBIN INST OF TECH
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