Method for increasing taxol content in cultivating Chinese yew

A taxus and paclitaxel technology, applied in the field of plants, can solve problems such as low content of paclitaxel, and achieve the effects of broad application prospects, alleviation of low content, and improvement of content

Inactive Publication Date: 2006-01-04
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to solve the problem of low paclitaxel content in the ab

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1 Soil cultivation

[0013] 1. Plant Material and Handling

[0014] The materials for field cultivation experiments were biennial Taxus x media purchased from Chengdu Plant Micropropagation Co., Ltd. in May 2003 and planted in the Taxus Experimental Garden, Shangcao Town, Lianshan County, Qingyuan City, Guangdong Province Medium (647m above sea level). Every plant was weighed for the first time when it was cultivated on May 26, 2003, and was dug out with roots on May 26, 2004. After washing the soil substrate with water, it was weighed again. The soil for planting is loose and fertile, suitable for the growth of yew.

[0015] The experiment set up 14 groups of Taxus×media small trees, 20 trees in each group, 12 groups were used for the experiment, and 2 groups were used as controls. Each experimental group received different doses of lanthanum chloride (see Table 1).

[0016] heavy metal salt

[0017] 2 results:

[0018] After cultivating for thre...

Embodiment 2

[0021] Example 2 Hydroponics

[0022] 1. Plant Material and Handling

[0023] The biennial Taxus×media saplings used in the hydroponic experiments were purchased from Chengdu Plant Micropropagation Co., Ltd. in May 2003 and planted in Shangcao, Lianshan County, Qingyuan City, Guangdong Province In the Taxus Experimental Garden in the town (647m above sea level). All seedlings used in experiments were screened for similar height and approximate biomass. The soil matrix in the root system was rinsed with double distilled water and handled with as little root damage as possible. Then the plants are transferred to 32 liters (17 (high) * 65 (long) * 29 (wide) centimeters) in the plastic container that is filled with corresponding treatment nutrient solution, in the medium, use air pump to pass through continuously, evenly with gas, Ten holes with a diameter of 6 cm were perforated on the plastic plate of the medium plastic container. Each plant was fixed in a long hole with a r...

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PUM

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Abstract

The present invention relates to cultivation method of increasing taxol content in Chinese yew. The method is to apply lanthanum containing compound as fertilizer additive under proper Chinese yew growing condition. In the root part of soil cultivated Chinese yew, lanthanum containing compound in 0.00015-0.0015 mole is applied for each plant, or, in the water culture liquid for Chinese yew, lanthanum containing compound is added to La ion concentration of 0.001-0.01 mol/L. The present invention can raise taxol content in Chinese yew and increase yield of taxane compounds with extremely high economic value. The present invention has wide application foreground.

Description

technical field [0001] The invention relates to the field of plants, in particular to a method for increasing the paclitaxel content in the cultivated yew. Background technique [0002] Taxus spp., commonly known as "water pine", is a national first-class protected plant, containing taxane compounds, and its secondary metabolite paclitaxel is a specific drug for the treatment of breast cancer and ovarian cancer, and it is effective for lung cancer, Kaposi's sarcoma It is also a broad-spectrum and highly active anticancer drug recognized in the world today. It can specifically promote the polymerization of tubulin and block cell division. It is a powerful tool for cytological research. The content of paclitaxel in the slow-growing yew is very low, the cost of extraction and separation is very high, and the price is very expensive. The increasing demand for clinical and scientific research has led to the disappearance of yew in many places, resulting in the scarcity of origin...

Claims

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Application Information

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IPC IPC(8): A01G7/00A01G17/00
Inventor 王昌伟彭少麟李鸣光李晓龙
Owner SUN YAT SEN UNIV
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