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Production of tricoderma using solid fermentation of grape peel and stillage

A solid-state fermentation, grape skin pomace technology, applied in applications, biocides, fungi, etc.

Inactive Publication Date: 2006-01-11
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And the method of producing Trichoderma biocontrol agent with grape pomace and distiller's grains as substratum has not yet been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Raw material pretreatment: Use screening equipment to separate the grape seeds from the grape pomace that has just left the factory, and dry or dry the seeded grape pomace as soon as possible to avoid mold and deterioration. Distillation is used to process the lees of the wine and recover the alcohol.

[0015] Liquid seed preparation: add 3 times of water to the processed distiller's grains, and adjust its pH value to 6.0 with sodium hydroxide. Sterilize at 121°C for 15 minutes, inoculate Trichoderma spores, shake the flask at 200 rpm at 30°C, and cultivate for 24 hours to obtain liquid seeds.

[0016] Fermentation product: take by weighing 20 kg of dried grape pomace after seed removal, add 30 kg of distiller's grains diluted 5 times (pre-adjusted to neutral pH), then inoculate 3 liters of Trichoderma liquid seeds, and stir well. Cultivate for 10 days in a solid-state fermentation reactor at 30°C. After the cultivated materials are dried, stabilizers and additives ar...

Embodiment 2

[0018] Liquid seed preparation: add 3 times of water to the processed distiller's grains, and adjust its pH value to 6.0 with sodium hydroxide. Sterilize at 121° C. for 15 minutes, inoculate Trichoderma spores, and culture in a stirred submerged fermentation reactor at 30° C. for 24 hours with aerobic consumption to obtain liquid seeds.

[0019] Fermented product: Weigh 20 kg of dried grape pomace after deseeding, add 35 liters of Trichoderma liquid seeds, and stir well. Cultivate for 10 days in a solid-state fermentation reactor at 30°C. After the cultivated materials are dried, stabilizers and additives are added, crushed and packaged.

Embodiment 3

[0021] After removing the seeds, take 20 kilograms of dried grape pomace, add 35 kilograms of 5 times diluted distiller's grains (adjust pH to neutrality in advance), inoculate 3 kilograms of Trichoderma solid-state fermentation products, and fully stir evenly. Cultivate for 10 days in a solid-state fermentation reactor at 30°C. After the cultivated materials are dried, stabilizers and additives are added, crushed and packaged.

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Abstract

A press for preparing the trichoderma used to prevent and treating multiple fungal diseases of plant includes such steps as screening the dregs and peels of grape to recover grape seeds, distilling the distillers' grains to recover alcohol, diluting the distillers' grains, mixing it with dregs and peels of grape to obtain culture medium for solid fermenting, sterilizing, inoculating trichoderma, solid fermenting, drying adding stabilizer and assistant and pulverizing.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides. technical background [0002] A large amount of grape pomace is produced in the wine production process, and these grape pomace are mainly composed of grape skins, grape seeds, stems and leaves. If not handled properly, it will cause serious pollution to the environment. Recent studies have shown that grape seeds can be used to produce high value-added products such as grape seed oil and antioxidants (proanthocyanidins), while grape skins, stems and leaves are used as feed (strict testing of pesticide residues is required) , most of which are processed by composting. These dregs contain high content of cellulose substances and a small amount of glucose, fructose, organic acids and other polysaccharides, which can provide good nutrition for microbial growth and are one of the ideal sources of nutrition for microbial resource development. [0003] Trichoderma sp. is widely distribut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04C12R1/885
Inventor 白志辉郝春博金波王晓谊张占营张洪勋
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI