Method of fast detecting colibacillus in beer using paper

Abstract

A method for quickly testing the intestinal flora in beer by test paper includes preparing said test paper and testing. It features that said culture medium contains proportionally water, lactose, peptone, sodium chloride dipotassium hydrogen phosphate, beef extract, agar, bromocresol purple and TTC. Its advantages are high speed, sensitivity and correctness, and low cost.

Description

technical field [0001] The invention relates to a method for rapidly detecting coliform bacteria in beer by using a paper sheet. Background technique [0002] The detection of coliform bacteria is an important part of food hygiene detection indicators. Coliform bacteria are normal intestinal parasites of humans and animals, without pathogenicity. From the perspective of food safety, food should not contain human pathogenic bacteria, otherwise the consequences of this food entering the market will be disastrous. However, it is very difficult to detect pathogenic bacteria, especially in general food production units, while the detection of coliform bacteria is relatively simple. When people detect the presence of coliform bacteria in food, it can be judged that the food has been contaminated by human or animal feces, and it is inferred that it may have been contaminated by human pathogenic bacteria, so the food cannot be eaten. [0003] The disk method is a method developed...

AI Technical Summary

Problems solved by technology

However, the disadvantage of this method is that the preparation of the medium is cumbersome, the operation is complicated, the entire detection cycle is long, and the detection results are seriously lagging behind. A detection cycle takes 72 hours, which seriously restricts the control of microbial indicators in the production process and the delivery of finished wine.