Method for preparing anti-hepatitis active part from swertia main pharmaceutical plant

A technology of medicinal plants and swedia, which is applied in the directions of drug combinations, pharmaceutical formulations, plant raw materials, etc., can solve the problems of undiscovered, undiscovered products, hindering the development of high-tech innovative drugs for anti-hepatitis active parts, etc. Simple, high extraction rate, good repeatability

Inactive Publication Date: 2006-02-08
青海普兰特药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method of extracting anti-hepatitis active parts in the main medicinal plants of Swertia genus has not been reported so far, and no actual products have been found in use.
Therefore, it directly hinders the development of high-tech innovative drugs based on this type of anti-hepatitis active site

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Take by weighing 100g of the dry whole herb of Swertia chinensis (S.mussotii) plant in western Sichuan, crush it (≤3mm), and use 850ml of 95% methanol aqueous solution to reflux extract three times at 70°C, each time for 2 to 4 hours, and each dosage is respectively 400ml, 300ml, 150ml, filter, recover methanol, and add appropriate amount of water to the system to obtain 100ml of cloudy solution, put it at room temperature or refrigerated (0-4°C) for 24 hours, centrifuge to obtain a clear solution for later use.

[0016] Weigh a certain amount of D-101 adsorption resin, swell overnight with an appropriate amount of ethanol, and then gradually transition to the water phase. Accurately measure 100ml of wet resin and transfer it to a column with an inner diameter of 1.5cm, and slowly flow 100ml of extract solution through the resin bed at a flow rate of 0.5-3BV / h. Then slowly wash the resin column with 50ml-500ml of water. Slowly elute with 100ml of 10%-70% methanol solut...

Embodiment 2

[0018] Take by weighing 100g of the dry whole plant of Swertia swerf (S. franchetiana), crush it (≤3mm), and use 850ml of 70%~95% ethanol aqueous solution to reflux extract three times at 50°C, each time for 3~5 hours, each time The dosage is 400ml, 300ml, 150ml each time, filter, recover ethanol, and add appropriate amount of water to the system to get 100ml of cloudy solution, put it at room temperature or refrigerated (0-4°C) for 24 hours, filter to get a clear solution spare.

[0019] Weigh a certain amount of D-101 adsorption resin, swell overnight with an appropriate amount of ethanol, and then gradually transition to the water phase. Accurately measure 100ml of wet resin and transfer it to a column with an inner diameter of 1.5cm, and slowly flow 100ml of extract solution through the resin bed at a flow rate of 0.5-3BV / h. Then slowly wash the resin column with 50ml-500ml of water. Slowly elute with 100ml of 10-70% ethanol solution at a flow rate of 0.5-2BV / h, collect ...

Embodiment 3

[0021] Weigh 100g of the dried whole herb of Swertia japonica (S.racemosa), crush it (≤3mm), and extract it with 850ml of aqueous solution at 100°C for three times under reflux, each time for 2-3 hours, and the dosage for each time is 400ml and 300ml, respectively. 150ml, filter, recover water under reduced pressure, and add an appropriate amount of water to the system to obtain 100ml of cloudy solution, place it at room temperature or refrigerated (0-4°C) for 24 hours, centrifuge to obtain a clear solution for later use.

[0022] Weigh a certain amount of D-101 adsorption resin, swell overnight with an appropriate amount of ethanol, and then gradually transition to the water phase. Accurately measure 100ml of wet resin and transfer it to a column with an inner diameter of 1.5cm, and slowly flow 100ml of extract solution through the resin bed at a flow rate of 0.5-3BV / h. Then slowly wash the resin column with 50ml-500ml of water. Slowly elute with 100ml of 10-70% ethanol solu...

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PUM

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Abstract

The invention provides a method for preparing hepatitis-resisting active sites mainly from medicinal herbal Swertiamarin, which comprises: (1) disintegrating and extracting in water or water hydrophilic organic solvent through backflow, filtering to obtain merged concentration liquid, freezing, centrifugally filtrating, obtaining extract, (2) passing through macroscopic adsorption resin columns, (3) water scrubbing the resin columns, purging with 10-70% ethanol or methanol, collecting the eluent and concentrating.

Description

technical field [0001] The invention relates to a method for preparing anti-hepatitis active parts from the main medicinal plants of the genus Swertia, in particular extracting with a hydrophilic solvent and enriching the anti-hepatitis activity of the main medicinal plants of the genus Swertia with a macroporous adsorption resin Process method for industrial production of partial products. Background technique [0002] The main medicinal plants in the genus Swertia are Swertia clam (S.franchetia), Swertia in western Sichuan (S.mussotii), Swertia in India (S.cliralyita), and Swertia in Pulan (S. .purpurascens) etc. are commonly used medicinal materials used in the Tibetan medicine system for the treatment of diseases of the liver and gallbladder system. The main research data show that Swertia is rich in triterpenes, bitters, flavonoids and ketones 【1，3】 [1, Drug Standards of the Ministry of Health of the People's Republic of China, Tibetan Medicine Volume 1, 1992; 6, 28; ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/51A61K31/7048A61P1/16A61P31/12
Inventor 孙洪发纪兰菊代冬海
Owner 青海普兰特药业有限公司
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