Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extracting and purifying high content lutein crystal from plant oil resin

A vegetable oil and lutein technology, applied in the field of phytochemistry, can solve the problems of high viscosity of organic solvents, difficult separation and purification operations, and difficult solvents, and achieve the effect of low cost and easy separation operation process

Active Publication Date: 2006-02-15
ZHE JIANG MEDICINE CO LTD XINCHANG PHARMA FAB
View PDF6 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] In short, the methods described in the above patents have the following disadvantages: 1) some organic solvents are used in the production process, and these solvents are difficult or impossible to completely remove, resulting in product lutein crystals that are not suitable for human consumption; 2) due to the use of The viscosity of the organic solvent obtained is relatively large, and the operation in the separation and purification process is relatively difficult. In order to obtain high-content crystals, multiple crystallization processes are required, which is not suitable for industrialized production; 3) the yield of lutein is low, due to the process Many steps are involved in the process, resulting in low product yield, only about 50%

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1000g marigold oleoresin (total lutein content 16.3%) is mixed with 1000mL ethanol, is heated up to 75 ℃, stirs until forming a kind of uniform flowing solution, slowly adds dropwise 375ml45% NaOH solution while stirring, and the dropping time is 60 Minutes, saponolysis reaction at this temperature for 4 hours.

[0037] Cool the reaction solution to 60°C, adjust its pH to 6-7 with 25% acetic acid aqueous solution, stir for 10 minutes, add 2000mL deionized water and 400mL ethanol, keep the solution temperature at 65°C during the dilution process, and stir slowly for 30 minutes . The resulting crystals are separated by centrifugation, which is relatively easy and can be completed within 10 minutes. The collected crystals were washed with hot water at 75°C for 2-3 times until the eluate was almost colorless, finally rinsed with absolute ethanol, and then vacuum-dried at 40°C until the weight loss on drying was less than 5%.

[0038] Finally obtain 150.4g finished product...

Embodiment 2

[0041] Using the same raw materials as in Example 1, using the same treatment method, except that the solution after saponolysis is not neutralized, but a mixed solution of 2000mL deionized water and 400mL ethanol at 65°C is directly added to the reaction solution , after stirring at a slow speed for 30 minutes, use the centrifuge same as in Example 1 to separate the crystals, the operation is much more difficult, and the operation process of this step will take more than 30 minutes. Subsequent processing and detection methods are the same as in Example 1.

[0042] The total yield of the final product lutein is 82%.

Embodiment 3

[0044] Mix 1Kg marigold oleoresin (total lutein content is 14.5%) with 2000mL ethanol, heat up to 60°C, stir to form a uniform flow system, then add 500mL 45% KOH aqueous solution dropwise, heat up to 75°C, and saponify for 4 hours . After the saponification reaction is completed, the temperature of the solution is lowered to 60°C, neutralized with 25% hydrochloric acid aqueous solution to make the pH of the mixture about 6.0-6.5, and after stirring for half an hour, dilute the reaction solution with a mixture of 2000mL deionized water and 800mL ethanol to maintain the temperature Stir at a slow speed for 0.5 hours at about 65°C, and you can see the generation of lutein crystals. Use the same centrifuge as in Example 1 to separate the solids. The crystals are also relatively easy to separate. The centrifugation process takes about 15 minutes.

[0045] Wash the collected filter cake with hot water at 75°C for 2-3 times until the mother liquor is almost colorless. After washing ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for superior edible high-content lutein crystal from vegetable oil resin without other organic solvent except edible alcohols. Wherein, neutralizing the saponified mixed solution to remove the water-soluble and oil-soluble impurity by hot water and alcohol respectively. The final product content and yield can achieve 90% and 80% respectively. This method is fit to industrial production.

Description

Technical field: [0001] The invention belongs to the technical field of phytochemistry. In particular, it relates to a method for separating and purifying edible-grade high-content lutein crystals from vegetable oleoresins. Background technique: [0002] Carotenoids are a class of yellow to red substances widely found in nature. In different fruits and vegetables, there are different kinds of carotenoids, such as β-carotene in carrots, lutein in marigold flowers, zeaxanthin in strawberries, lycopene in tomatoes, capsanthin in capsicum plants and capsicum content are higher. However, there are more carotenoids in some dark fruits and vegetables, egg yolks, some fish shells, birds, seaweed and bacteria, and the content of lutein is also higher. [0003] Several animal and human trials in recent years have shown the beneficial effects of carotenoids. In general, carotenoids may be divided into two subcategories, lutein or oxygen-containing carotenoids with relatively strong...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24
CPCC07C403/24
Inventor 许新德邵斌周迪叶双明王燕峰陈伯秋
Owner ZHE JIANG MEDICINE CO LTD XINCHANG PHARMA FAB
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com