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Microorganism fertilizer and its preparation method

A technology of microbial organic fertilizer and microbial strain, which is applied in the direction of organic fertilizer, can solve the problems of single strain and single function, and achieve the effect of improving utilization rate, scientific formula, and improving energy utilization rate

Inactive Publication Date: 2006-04-26
广东金饭碗环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to overcome the defects of single strain and single function used in existing microbial fertilizers, and provide a microbial fertilizer and a preparation method thereof. The microbial fertilizer of the present invention is a liquid multifunctional microbial organic fertilizer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Culture of Saccharomyces cerevisiae

[0040] ①Expansion culture at one time

[0041] Medium: peptone 10.0g, glucose 10.0g, KH 2 PO 4 3.0g, K 2 HPO 4 5.0g, MgSO 4 ·7H 2 O 2.0g, add water to 1 liter;

[0042] Mix the raw materials according to the above ratio, heat to dissolve, adjust the pH value to 5-6, autoclave at 121°C for 20 minutes, cool to 30°C, and pack in 500ml Erlenmeyer flasks, 200-250ml per bottle.

[0043] Insert Saccharomyces cerevisiae strains, each triangle flask is connected with 10-20ml strains, cultivated at a constant temperature, the temperature is 28-30°C, and the cultivation time is 48h;

[0044] ②Secondary expansion training

[0045] Medium: brown sugar 1000g, urea 300g, KH 2 PO 4 100g, add water to 10 liters;

[0046] Mix the raw materials according to the above ratio, heat to dissolve and boil for 20 minutes, adjust the pH value to 5-6, and then culture 1 liter of the expanded culture at a temperature of 28-30°C for 72 hou...

Embodiment 2

[0047] Embodiment 2 Bacillus subtilis culture

[0048] ①Expansion culture at one time

[0049] Medium: peptone 10g, beef extract 3g, NaCl 5g, add distilled water to 1 liter;

[0050] Weigh the raw materials according to the above ratio, mix and heat to dissolve, adjust the pH value to 7.0, autoclave at 121°C for 20 minutes, cool to 35°C, and pack in 500ml Erlenmeyer flasks, 200-250ml per bottle. Bacillus subtilis strains were inoculated, and each triangular flask was inoculated with 10-20ml strains for cultivation at a temperature of 33-35°C and a cultivation time of 48h.

[0051] ②Secondary expansion training

[0052] Medium: brown sugar 1000g, soybean meal 100g, add water to 10 liters;

[0053] Weigh the raw materials according to the above ratio, heat, dissolve and boil for 20 minutes, cool to 33-35°C, insert 1 liter of expanded culture strains once, and cultivate for 72 hours.

Embodiment 3

[0054] Example 3 Fermentation production

[0055] (1) The formula in parts by weight of raw materials is: brown sugar 100kg, fishmeal 85kg, bran 15kg, urea 50kg, KH 2 PO 4 25kg, KNO3 10kg, water 1000kg;

[0056] (2) Mix the raw materials according to the above ratio, heat and boil for 20 minutes, and cool;

[0057] (3) Yeast fermentation: control the temperature at 28-30° C., insert the Saccharomyces cerevisiae production strain cultivated in Example 1 according to the inoculation amount accounting for 10% of the raw material weight, and ferment for 72 hours until the residual sugar is divided into zero;

[0058] (4) Bacillus subtilis fermentation: After the above-mentioned fermentation process is finished, be warmed up to 33-35 ℃, the Bacillus subtilis production bacterial classification prepared in embodiment 2 accounts for 10% of raw material weight by inoculum insertion, carries out aerobic fermentation, One week to complete the fermentation process.

[0059] Then do...

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Abstract

The microbial organic fertilizer is prepared with brown sugar, fish meal, wheat bran, urea, KH2PO4, KNO3, and water in certain weight proportion, and through inoculating Saccharomyces cerevisiae and Bacillus subtilis and fermenting. It is one kind of multifunctional liquid organic fertilizer and may be applied to foliage or soil. It contains comprehensive nutrients and can raise the utilization of nitrogen fertilizer, phosphate fertilizer and potash fertilizer.

Description

technical field [0001] The invention relates to a microbial organic fertilizer and a preparation method thereof. Background technique [0002] Microbial fertilizer is a type of fertilizer that uses microbial life activities to obtain specific fertilizer effects for crops. In recent years, with the continuous development of science and technology, some fertilizers produced by microbial fermentation have been produced rapidly at home and abroad. The microorganisms are mainly nitrogen-fixing bacteria, phosphorus bacteria, and potassium bacteria, as well as a large number of beneficial strains screened from soil. At present, most of the microbial fertilizers are mainly microbial agents, which use the existing nutrients to cultivate a large number of microbial cells, and then adsorb on a certain carrier. After use, a certain characteristic of microorganisms is used to decompose and utilize a specific nutrient in the soil that cannot be directly used by crops. For example, phosp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F11/08
Inventor 孙明坤
Owner 广东金饭碗环境科技有限公司
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