Formaldehyde repid test kit and its use

Abstract

Without need of additional instrument, the kit is capable of mensurating concentration range of formaldehyde contained in article rapidly; and minimum limit of detection is 0.8mg / Kg. The disclosed kit includes injector of active carbon, reaction liquid, test tube, and standard color grade. The reaction liquid is prepared from acetylacetone, glacial acetic acid, and ammonium acetate according to mol ratio per hundred milliliter 39:105:4000. The standard color grade is a color card in series of light yellow. The method includes steps: adding the reaction liquid into supernatant solution from dipped specimen to be tested; after 6 min, comparison is carried out between developed color and standard color grade; determining content range of formaldehyde. Features are: simple operation, quick and sensitive and widely available to test in site.

Description

technical field [0001] The invention belongs to a test kit for determination, and relates to a test kit for rapidly determining formaldehyde content in food and a determination method thereof, especially capable of rapid determination on the spot. Background technique [0002] Formaldehyde, molecular formula HCHO, colorless gas. Melting point -92°C, boiling point -21°C, relative density 0.815 (20 / 4°C). Has a strong pungent odor. Soluble in water, soluble in ethanol, ether and other organic solvents. An aqueous solution with a formaldehyde concentration between 37% and 40% is usually called formalin. Formaldehyde is a highly toxic protoplasmic poison that destroys biological cell proteins. It can combine with amino groups of proteins to denature and solidify proteins and disrupt cell metabolism. Simultaneously formaldehyde is carcinogenic and teratogenic substance, is one of potential strong mutagen. Long-term exposure to low doses of formaldehyde can cause gene mutation...

AI Technical Summary

Problems solved by technology

Among them, both high-performance liquid chromatography and chromotropic acid colorimetry require instrumental analysis, which is not conducive to on-site determination, and acetaldehyde interferes with chromotropic acid colorimetry.

The dinitrophenylhydrazine method is suitable for the determination of substances containing carbonyl groups, so substances with carbonyl groups will affect this method, and it is easy to cause misjudgment

The reagents required by the phenol reagent method are expensive, which is not conducive to popularization

The sensitivity of the silver mirror method is too low, and heating is required during the detection process, which is not conducive to rapid determination

There are following problems in the existing colorimetric method for measuring formaldehyde: some methods need to be equipped with a spectrophotometer; some methods often have a color in the aqueous solution after the sample is soaked, and at this time, after adding a color developer, the color will be affected. The judgment has a certain influence; some methods require heating and distillation of the sample, and some methods require heating in a water bath when the sample reacts with the detection solution

The above heating process is a waste of energy and does not meet the requirements of rapid detection

The sensitivity of the existing rapid detection method is not high, about 1.0mg / Kg

In short, there is no rapid determination kit for the determination of formaldehyde in food that is suitable for on-site detection requirements and the detection limit is 0.8mg / Kg.

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