Separation and purification of high purity hemoglobin and virus inactivation technology

Abstract

The present invention relates to a separation, purification and virus inactivation process of high-purity haematoglobin which can be used as substitute for red blood cell. Said process is characterized by including the following steps: under the condition of 0-4deg.C using natural red blood cell and making it undergo the process of haematolysis treatment to obtain primary haemolytic solution, adding proper quantity of antioxidant to regulate pH value of solution, making physical deoxidation and chemical deoxidation to fully remove oxygen existed in the haematoglobin, then adding excess antioxidant and adding saccharide compound as protecting agent, heating for 10-12hr at 60-80deg.C and continuously introducing inert gas, then making conventional filtration and ultrafiltration membrane treatment and regulating pH to 7.0-7.4.

Description

(1) Technical field: [0001] The invention relates to the separation and purification and virus inactivation process of hemoglobin, in particular to the separation and purification and virus inactivation process of high-purity hemoglobin used in red blood cell substitutes. (two) background technology: [0002] Although the traditional blood transfusion method has played an important role in medical rescue, there are also various problems, such as blood shortage, virus transmission, immune response, and the treatment of expired blood. In various clinical medical treatment and first aid, the requirements for the quality and quantity of blood transfusion are getting higher and higher, so the research and development and use of red blood cell substitutes have generally been paid attention to. Among various hemoglobin oxygen carriers, purified hemoglobin products are required as raw materials. The physical and chemical indicators of purified hemoglobin have a direct impact on the...

AI Technical Summary

Problems solved by technology

Although this document describes the research on conditions such as deoxidation, heating, and virus inactivation, most of the antioxidants used in the research are more toxic, and the key technology of adding excessive non-toxic antioxidants has not been solved, and the above methods The purity of the obtained hemoglobin is less than 99%, and the yield is only about 80%

Yang Chengmin and others published a patent (patent No. CN01108643.2) in 2001 and pointed out that heating at 50-60°C can effectively separate and purify hemoglobin, but the yield of this process is only 80%, and the purpose of virus inactivation cannot be achieved, so it must Further improve its process in order to make the process route more reasonable and obtain higher quality and yield of purified hemoglobin

Images