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Production technology of phycoerythrin and crystal and product thereof

A phycoerythrin and crystal technology, applied in the field of crystal manufacturing process and products, and protein, can solve the problems of high fluorescence activity, can not be stored for a long time, easy to lose biological activity, etc., and achieve the effect of high fluorescence activity and small requirements for preservation conditions.

Inactive Publication Date: 2006-06-14
骆建华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention also aims at the disadvantages that the protein in the prior art is in the state of freeze-dried powder or precipitated, cannot be preserved for a long time, its biological activity is easily lost, it cannot be used for a long time, and its fluorescence activity is low; it provides a long-term preservation and the required preservation conditions Phycoerythrin crystals with small requirement and high fluorescence activity

Method used

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  • Production technology of phycoerythrin and crystal and product thereof
  • Production technology of phycoerythrin and crystal and product thereof
  • Production technology of phycoerythrin and crystal and product thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0117] 1. Preparation of phycoerythrin

[0118] cell disruption

[0119] Weigh an appropriate amount of phycoerythrin raw material (such as seaweed, polytubule and other algae), wash it twice with distilled water, grind it with a tissue crusher at 0°C, put it into a beaker, add pH value 6.5, concentration 0.005 mol / liters of phosphate-buffered saline (PBS), and stir well. Ultrasonic crushing at 1200W in an ice bath for 60 minutes, the crushed crude liquid was left to stand at low temperature (0°C) for 10 hours, and then filtered three times with at least three layers of gauze to remove large cell tissue fragments, and the filtrate was placed in a pre- In a cold high-speed refrigerated centrifuge, centrifuge at 8,000 rpm at 0°C for 60 minutes, remove the precipitate, and collect the supernatant;

[0120] Ammonium sulfate graded salting out

[0121] Add solid ammonium sulfate to the supernatant to make it reach 30% saturation, let stand at 10°C for 10 hours, centrifuge at 60...

Embodiment 2

[0142] 1. Preparation of phycoerythrin

[0143] cell disruption

[0144] Weigh an appropriate amount of phycoerythrin raw material (such as laver, polytubule and other algae), wash it twice with distilled water, grind it with a tissue crusher at 2°C, put it into a beaker, add pH value 6.8, concentration 0.005 mol / liters of phosphate-buffered saline (PBS), and stir well. Ultrasonic crushing at 1200W in an ice bath for 60 minutes, the crushed crude liquid was left to stand at low temperature (2°C) for 10 hours, and then filtered three times with at least three layers of gauze to remove large cell tissue fragments, and the filtrate was placed in a pre- In a cold high-speed refrigerated centrifuge, centrifuge at 8,000 rpm at 2°C for 60 minutes, remove the precipitate, and collect the supernatant;

[0145] Ammonium sulfate graded salting out

[0146] Add solid ammonium sulfate to the supernatant to make it reach 30% saturation, let stand at 2°C for 10 hours, centrifuge at 6000 ...

Embodiment 3

[0167] 1. Preparation of phycoerythrin

[0168] cell disruption

[0169] Weigh an appropriate amount of phycoerythrin raw material (such as laver, polytubule and other algae), wash it twice with distilled water, grind it with a tissue crusher at low temperature (5°C), put it into a beaker, add pH value 7.0, concentration 0.005 mol / L phosphate buffered saline (PBS), stir well. Ultrasonic crushing at 1500W in an ice bath for 45 minutes, the crushed crude liquid was left to stand at low temperature (5°C) for 18 hours, and then filtered three times with at least three layers of gauze to remove large cell tissue fragments, and the filtrate was placed in a pre- In a cold high-speed refrigerated centrifuge, centrifuge at 8,000 rpm at 4°C for 45 minutes, remove the precipitate, and collect the supernatant;

[0170] Ammonium sulfate graded salting out

[0171] Add solid ammonium sulfate to the supernatant to make it reach 25% saturation, let stand at 5°C for 18 hours, centrifuge at ...

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Abstract

The present invention relates to a protein, crystal preparation process and its product. In particular, it relates to a phycoerythrin which is extracted from algae under the general condition and can emit strong fluorescence, and can convert it into crystal form, It can be extensively used in the fields of scientific research, medicine detection and telecommunication, etc.

Description

technical field [0001] The present invention relates to a protein, crystal manufacturing process and products, especially to the phycoerythrin that can emit strong fluorescence extracted from algae under normal conditions and transform it into crystal form, and is used in scientific research, medical treatment, detection, Applications in telecommunications and other fields. Background technique [0002] As early as the beginning of the last century, it was reported abroad that there were red, violet and blue proteins with strong fluorescence in cyanobacteria and red algae. In 1910, Kylin named these pigment proteins as "Phycochromo-proteins" for the first time. This light-harvesting pigment protein that appears in large quantities in red algae, blue-green algae and cryptophyta is the phycobiliproteins (Phycobiliproteins, PBP) that is currently in urgent need of development, which mainly includes phycoerythrin (Phycoerythrin, PE), phycocyanin (Phycocyanin, PC), phycoerythro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/405C07K1/36
Inventor 骆建华刘维国刘俊
Owner 骆建华
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