Production technology of allophycocyanin and crystal and product thereof

A technology of allophycocyanin and crystals, which is applied in the fields of medical treatment, testing, scientific research, allophycocyanin, and telecommunications. It can solve the problems of low fluorescence activity, small storage conditions, and inability to use for a long time to achieve fluorescence. The effect of high activity and low requirement for storage conditions

Active Publication Date: 2006-06-14
福建省神六保健食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention also aims at the disadvantages that the protein in the prior art is in the freeze-dried powder or precipitated state, its biological activity is easily lost, it cannot be used for a long time, its side chain groups are easily exposed, its physicochemical properties and biochemical properties are easy to change, and its fluorescence activity is low; Provide an allophycocyanin crystal that can be stored for a long time, requires less storage conditions, and has high fluorescence activity

Method used

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  • Production technology of allophycocyanin and crystal and product thereof
  • Production technology of allophycocyanin and crystal and product thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0107] 1. Preparation of allophycocyanin

[0108] Cell disruption

[0109] Weigh Spirulina platensis into a beaker, wash it twice with distilled water, add a phosphate buffer with a pH of 5.8 and a concentration of 0.005 mol / L, and stir evenly. In an ice bath (0°C), 1800W ultrasonically broken for 30 minutes, the broken algae liquid is placed in a pre-cooled high-speed refrigerated centrifuge, and centrifuged at 8000 revolutions per minute (rpm) at 0°C for 60 minutes. The precipitate is removed and collected. Clear liquid

[0110] Salting out

[0111] Add solid ammonium sulfate to the supernatant to reach 25% saturation, let it stand at 0°C for 10 hours, and centrifuge at 6000 revolutions per minute (rpm) at 0°C for 60 minutes, and discard the precipitate. Continue to add solid ammonium sulfate to the supernatant to 80% saturation, let stand at low temperature (0°C) for 10 hours, and centrifuge at 6000 revolutions per minute (rpm) at 0°C for 60 minutes. Discard the supernatant and...

Embodiment 2

[0133] 1. Preparation of allophycocyanin

[0134] Cell disruption

[0135] Weigh Spirulina platensis into a beaker, wash it twice with distilled water, add a phosphate buffer with a pH of 6.8 and a concentration of 0.005 mol / L, and stir evenly. In an ice bath (0°C), 1800W ultrasonically crushed for 40 minutes, the crushed algae solution was placed in a pre-cooled high-speed refrigerated centrifuge, centrifuged at 8000 revolutions per minute (rpm) at 2°C for 45 minutes, the precipitate was removed and collected. Clear liquid

[0136] Salting out

[0137] Add solid ammonium sulfate to the supernatant to reach 25% saturation, let it stand for 12 hours at a temperature of 2°C, centrifuge at 8000 revolutions per minute (rpm) at 2°C for 40 minutes, and discard the precipitate. Continue to add solid ammonium sulfate to the supernatant to 80% saturation, let stand at low temperature (2°C) for 10 hours, centrifuge at 8000 revolutions per minute (rpm) at 2°C for 45 minutes, discard the supe...

Embodiment 3

[0159] 1. Preparation of allophycocyanin

[0160] Cell disruption

[0161] Weigh Spirulina platensis into a beaker, wash it twice with distilled water, add a phosphate buffer with a pH of 7.1 and a concentration of 0.005 mol / L, and stir evenly. In an ice bath (0°C), 1800W ultrasonically crushed for 40 minutes, the crushed algae liquid was placed in a pre-cooled high-speed refrigerated centrifuge, centrifuged at 8000 revolutions per minute (rpm) at 4°C for 45 minutes, the precipitate was removed and collected. Clear liquid

[0162] Salting out

[0163]Add solid ammonium sulfate to the supernatant to reach 30% saturation, let it stand at 5°C for 16 hours, centrifuge at 7000 revolutions per minute (rpm) at 4°C for 40 minutes, and discard the precipitate. Continue to add solid ammonium sulfate to the supernatant to 80% saturation, let stand at low temperature (5°C) for 18 hours, and centrifuge at 6000 revolutions per minute (rpm) at 4°C for 40 minutes. Discard the supernatant and use ...

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Abstract

The present invention relates to a protein, crystal preparation process and its product, in particular, it relates to an allophycocyanin which is extracted from algae under the general condition and can emit strong fluorescence, and can convert it into crystal form. It can be extensively used in the fields of scientific research, medicine, detection and telecommunication, etc.

Description

Technical field [0001] The present invention relates to a protein and crystal manufacturing process and products, in particular to allophycocyanin that is extracted from algae under normal conditions and can emit strong fluorescence and converts it into a crystal form, and it is used in scientific research, medical treatment, etc. Applications in the fields of detection, information and telecommunications. Background technique [0002] As early as the beginning of the last century, foreign countries have reported the existence of red, violet and blue proteins with strong fluorescence in cyanobacteria and red algae. In 1910, Kylin named these pigment proteins "Phycochromo-proteins" for the first time. This kind of light-harvesting pigment protein, which appears in large quantities in red algae, blue-green algae and cryptophyte, is the phycobiliprotein (PBP) that needs to be developed urgently. It mainly includes phycoerythrin (Phycoerythrin, PE) and phycocyanin. (Phycocyanin, PC),...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/405C07K1/36
Inventor 骆建华刘维国刘俊
Owner 福建省神六保健食品有限公司
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