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Method of crossing flower color genotypes

A technology of pigment gene and hybridization method, which is applied in the field of hybridization of flowers, new plants and their acquisition methods, and genotypes, and can solve problems such as doubts about the separation ratio of offspring, mutations, and genetic recombination

Inactive Publication Date: 2006-06-28
KAGOSHIMA TLO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0020] However, the breeding methods based on the genotype of the flower color itself still have many unclear points about the segregation of the flower color of the progeny, so there are still many problems in the practical application of these breeding methods
At the same time, as disclosed in Non-Patent Document 6, the inheritance of anthocyanins of geraniums (Pelargonium × hortorum) represented by E1 / e1 and E2 / e2 has doubts about the segregation ratio of offspring, so it has not been applied to Practice
As for the method in the patent document, there is also the following problem: in order to produce a new flower color, gene recombination, mutation (such as radiation mutation) etc. are required

Method used

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  • Method of crossing flower color genotypes
  • Method of crossing flower color genotypes
  • Method of crossing flower color genotypes

Examples

Experimental program
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Effect test

Embodiment 1

[0057] Collect petals, peels and leaves, and for petals and sepals, etc., cut out colored parts, parts of the same color, and white parts of the panchromatic type or lacy type (including double flowers), and weigh them accurately . Thereafter, an acidic solvent is added, such as 0.5N to 2N hydrochloric acid (0.5N to 2N HCl), and then the anthocyanins are extracted. The extraction is based on literature (Uddin et al.: J.Jpn.Soc.Hort.Sci., 71:40-47, 2002; Wang et al.: J.Plant Res., 114:213-221, 2001; Naotaka Matsuzoe and others 5 persons: Engakuzatsu, 68: 138-145, 1999). The extract is filtered with cotton, and then the filtrate is heated at 95° C. to 100° C. to perform hydrolysis to obtain a solution containing 1 to 6 types of anthocyanins. The hydrolysis is carried out according to literature (Uddin et al.: J.Jpn.Soc.Hort.Sci., 71:40-47, 2002). After the reaction was completed, the reaction mixture was filtered with a membrane filter, and then analyzed using an HPLC instrum...

Embodiment 3

[0064] With the eustoma's S shown in Table 3 1 As parents, they are self-pollinated and segregated to obtain S 2 Generation, detection of the S 2 Generation to determine the pigment genotypes of different lines. The results are shown in Table 3.

[0065] pigment phenotype

[0066] As Table 3 clearly shows, the G2D3B25F lines (white, red-white, cream or pink flowers) with only Pgn pigments were obtained from ddeeH F h F PgPgCyCyDpDp Pigment genotype. The G2D3B27Y line with only Cyn pigments (red-white or pink flowers) was obtained from ddeeH T h T pgpgCyCyDpDp Pigment genotype. The G2D3B26B line without pigment (white flowers) as a blank phenotype was obtained from ddeeH F h F pgpgCyCyDpDp Pigment genotype. J5A2H16B line (red-purple flowers) with CynDpn pigment phenotype was obtained from ddeeH O h O pgpgCyCyDpDp Pigment genotype. J5A2H110C1A line (purple flowers) with Dpn pigmented phenotype was obtained from ddeeH D h D pgpgCyCyDpDp Pigment genotype. ...

Embodiment 4

[0068] When red and white flowers with Pgn phenotype (G2D3B25F line, ddeeH F h F PgPgCyCyDpDp pigment genotype) and red-white flowers with Cyn phenotype (G2D3B27Y line, ddeeH T h T pgpgCyCyDpDp pigment genotype) when cross-pollination, obtain the PgnCynDpn phenotype (ddeeH T h F PgpgCyCyDpDp pigment genotype) reddish-purple flowers. Non-Patent Document 6 (Kanai Kobayashi, Journal of Breeding Science, 48: 169-176, 1998) (which proposes a conjecture of a pigment phenotype) discloses the acquisition of flowers with a PgnCyn phenotype, but cannot explain the red flowers with a PgnCynDpn phenotype. Isolation of purple flowers.

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Abstract

The purpose of the present invention is to clarify the genetic characteristics of anthocyanin biosynthesis and the relationship between flower color inheritance and pigment genotype, so as to provide a hybridization method for flower color genotypes that can create new flower colors in practice. The present invention has discovered a new rule: the flower color genotype is related to the biosynthesis of flavonoids shown in the pathway of formula (I), and flavonoid 3′-hydroxylase (F3′H) and flavonoid 3′, 5′- The genetic characteristics of hydroxylase (F3', 5'H) are controlled by 5 multiple alleles. The result makes it possible to provide a method for creating new flower colors using genotypes D / d·E / e·HXHX·Pg / pg·Cy / cy·Dp / dp by which flower colors can be freely created according to pigment genotypes Without resorting to genetic recombination or mutations caused by radiation exposure, etc.

Description

technical field [0001] The present invention relates to a method of breeding flowering plants with new flower colors using the pigment genotypes of the flowering plants. More specifically, the present invention relates to new plants and methods of obtaining new plants including flowering plant flowers (ie, angiosperm flowers) and methods of crossing to alter their genotype. Furthermore, the present invention also relates to a method of utilizing a plant or a part of a plant obtained by breeding, wherein said breeding includes a sexual crossing stage. Furthermore, the present invention also relates to new plants and methods for obtaining them, ie to new flowering plants, such as angiosperms, especially flowers. Background technique [0002] Anthocyanins are a class of flavonoids widely present in flowers, fruits, leaves, etc. of plants, and are pigment glycosides involved in the coloring of red, purple, and blue. When anthocyanins are hydrolyzed by hydrochloric acid, they a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/1215
Inventor 桥本文雄坂田祐介
Owner KAGOSHIMA TLO
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