Jellyfish collagen and method for preparing the same

A technology of jellyfish collagen and collagen, which is applied to the preparation method of peptides, chemical instruments and methods, protein food components, etc., can solve the problems of large table salt, difficulty in salt recovery, and difficulty in acidic solution recycling, so as to facilitate storage, The effect of high purity

Inactive Publication Date: 2006-07-19
THE FIRST INST OF OCEANOGRAPHY SOA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many domestic literatures report the technology and process of extracting collagen from beef tendon, pig skin, pig cartilage, fish skin and fish scale, all of which use enzymatic hydrolysis and salting out extraction process. The problem with salting out extraction is that protease recovery is difficult , Difficulties in salt recovery and recycling of acidic solu...

Method used

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  • Jellyfish collagen and method for preparing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Take 120g of salted jellyfish, soak in 100ml of 0.2M sodium hydroxide solution for 2 hours, the pH of the solution after soaking is 6.5, ammonia gas will be generated during alkali washing, and the bubbles will be obvious, which can remove ammonia ions and impurities. The jellyfish skin changes from white opaque to transparent (similar to frozen dehydrated jellyfish), and the middle glue layer can be clearly seen. Remove the upper and lower cortex, slice the white middle layer and store in the freezer.

[0026] Take the above-mentioned medium rubber layer, grind it, add 20ml of 0.1M acetic acid, and add 600mg of pepsin (Huamei, Sina-American Biotec, pepsin 1:3000), stir and dissociate at room temperature for 30h.

[0027] Ultrafiltration membrane filter is used to control the membrane inlet pressure at 1-2kg / cm 2 , and adopt an external electric field of 10-70V / cm, intercept concentrated molecular weight at 100,000-300,000 Daltons of collagen, and its interception rate...

Embodiment

[0032] Embodiment (1)-(7) concrete process condition and result are shown in Table 1:

[0033]

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Abstract

The disclosed collagen extracted from jellyfish mesoglea belongs to white fiber solid or light-yellow solid powder with bulk density as 0.110-0.645g/ml, shows clear liquid after dissolving in acidic solution completely; has single-bond molecular weight as 100-300 thousands dalton, sugar content as 2.5-5%, amino acid content as 80-95%mg/100mg with 7.9-8.3% aspartic acid, 10.0-12.9% glutamic acid, 3.5-4.0% threonine, 3.5-3.9% valine, 2.2 -2.5% isoleucine, 3.4-3.8% lycine, and 2.0-2.7% hydroxylysine. Besides, the opposite preparation method comprises: (1) selecting salinized or fresh jellyfish; (2) pre-treating; (3) separating the mesoglea; (4) dissociating mesoglea; (5) ultrafiltrating, separating, and condensing. This invention has trapping rate as 95-98% and high product purity.

Description

technical field [0001] The invention relates to the improvement of marine biochemical raw materials, in particular to a jellyfish collagen and a preparation method thereof. It belongs to the technical field of biomedicine and chemical industry. Background technique [0002] Jellyfish, also known as jellyfish, is a coelenterate. Jellyfish are carnivorous, feeding on plankton, small crustaceans, polychaetes and even small fish. The body is hemispherical, and the diameter of the umbrella is generally 30 to 45 centimeters, and the largest one can reach 100 centimeters. Between the epidermis and stomach layer of jellyfish is the mesoglea, which is very developed and occupies almost the entire thickness of its body, which contains fibers and a small amount of cells derived from the ectoderm. The middle glue layer is hard and thick, and there are many small fibers in the middle glue layer that can be seen under the electron microscope. The main component in the middle glue laye...

Claims

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Application Information

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IPC IPC(8): C07K14/435C07K1/14A23J1/04
Inventor 石红旗
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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