Method for preparing hard-tissue low-temperature buried cold-ester

Abstract

This invention discloses a preparation method for embedding condensing ester at low temperature to hard tissues including: first of all cutting a bone tissue into slices of 1-2cm thick with a diamond-impregnated blade, then putting them into a stationary liquid to be kept, then dehydrating the cleaned sample in gradient with alcohol, then putting the dehydrated sample into an intermediated solution of penetration and then to a penetration solution for vacuumizing in intermission, after that, putting the penetrated tissue into an embedded bottle added with fresh embedding liquid to be polymerized in a refrigerator, then crushing the bottle to take out the block to be cut on a hard tissue slicer to be attached on a glass carrier to be dried and finally dying it .

Description

technical field

[0001] The invention relates to a preparation method of a hard tissue low-temperature embedding agent (condensate PMG), which is widely used in research in the fields of medicine, material science, biology and the like. Background technique

[0002] The development of hard tissue sections in foreign countries (mainly concentrated in the Nordic countries) was early, and this technology was carried out in many laboratories in the 1980s, and the conventional embedding method of methyl methacrylate (MMA) has been used (30 degrees ~ Polymerization at 40 degrees), methyl methacrylate (MMA) can be removed very easily and completely, and the dyeing result after film production is excellent, and the shape details are good (Erben 1997, Embedding of bone samples inmethylmethacrylate: an improved suitable for bonehistomorphometry, historychemistry , and immunohistochemistry. J Histochem Cytochem). It can be considered that methyl methacrylate...