Biological oxygen demand microbe sensor sensitive membrane activating process and automatic activating apparatus

Abstract

This invention is biochemical oxygen needing microorganism sensor sensitive membranes activation method and its auto active equipment. It relates to a microorganism sensor, it provides a manual active method used to BOD microorganism sensor sensitive membranes and auto activation method and its auto active equipment. When manual method is used, the membranes is soaked to fluorescence signal stable in GGA solution, then GGA solution is discharged and the membranes is aerated to the signal stable. The membranes are soaked with new GGA solution to dynamic balance, soak and aeration is done several times for multiple activations. The equipment is set with basin, pump, control circuit, active slot, liquid level pole and electromagnetic valve. Then auto method is used, the pump is soaked in the GGA solution, the membranes soaking and aeration time is controlled, then the membrane is dropped into the active slot, then the GGA solution in the water slot is taken into the active slot. The membrane is soaked into GGA solution and serration, then soaking and aeration is repeated.

Description

technical field [0001] The invention relates to a microbial sensor, in particular to an activation method and an automatic activation device which can be used for a biochemical oxygen demand (BOD) microbial sensor sensitive film. Background technique [0002] Biochemical oxygen demand (Biochemical Oxygen Demand, BOD) is one of the most commonly used and most important indicators in the process of water quality evaluation. The currently widely used 5-day biochemical oxygen demand standard dilution method is a standard method formulated by the American Public Health Association in 1936. However, the traditional measurement method not only requires time-consuming and complicated operation steps such as 5-day cultivation, but also the accuracy of the measurement has a lot to do with the proficiency and experience of the operator, which cannot realize real-time and online monitoring of environmental water samples. Therefore, research on BOD microbial sensors and related instrume...

AI Technical Summary

Problems solved by technology

It can be seen from the above that the activation of microbial pellicles is an indispensable step in the rapid assay of microbial sensors, and the preservation and activation methods of microbial pellicles similar to existing industry standards have the following disadvantages: (1) It takes a long time and requires 48h or even a few days

(2) After the biofilm is activated by PBS, it needs to continue to be installed on the surface of the electrode for activation. It can only be used after the signal is stable, which will affect the use of the sensing device and the continuous measurement of samples.

(3) If the bacterial film is used continuously for several hours, it needs to be replaced with a new film and reactivated, which will affect the continuity of the measurement and the reproducibility and stability of the measurement results

These tasks need to be done manually on the sensor, which is time-consuming, cumbersome, and occupies the sensing device, affecting the continuity and timeliness of detection

So far there is no automatic activation device for microbial sensing membranes specially used for biochemical oxygen demand (BOD) microbial sensors

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