Biological oxygen demand microbe sensor sensitive membrane activating process and automatic activating apparatus

A microbial sensor, biochemical oxygen demand technology, applied in biochemical equipment and methods, microbial determination/inspection, testing water, etc., can solve problems such as time-consuming, time-consuming, cumbersome, etc., to ensure reproducibility and stability performance, saving manpower and time, and improving work efficiency

Inactive Publication Date: 2006-08-30
XIAMEN UNIV
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  • Summary
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  • Application Information

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Problems solved by technology

It can be seen from the above that the activation of microbial pellicles is an indispensable step in the rapid assay of microbial sensors, and the preservation and activation methods of microbial pellicles similar to existing industry standards have the following disadvantages: (1) It takes a long time and requires 48h or even a few days
(2) After the biofilm is activated by PBS, it needs to continue to be installed on the surface of the electrode for activation. It can only be used after the signal is stable, which will affect the use of the sensing device and the continuo...

Method used

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  • Biological oxygen demand microbe sensor sensitive membrane activating process and automatic activating apparatus
  • Biological oxygen demand microbe sensor sensitive membrane activating process and automatic activating apparatus
  • Biological oxygen demand microbe sensor sensitive membrane activating process and automatic activating apparatus

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Embodiment 1

[0040] see figure 1 and 2 The said biochemical oxygen demand (BOD) microbial sensor of the present invention is a kind of optical fiber photochemical BOD microbial sensor. The optical fiber photochemical BOD microbial sensor sensitive film is composed of a transparent optical glass sheet 1, an oxygen sensing film 2 and a bacterial film 3. The oxygen sensing film 2 is set in the middle of the upper surface of the transparent optical glass sheet 1, and the bacterial film 3 is fixed on the oxygen sensor on film 2. The biofilm 3 adopts the mixed biofilm. The size of the transparent optical glass sheet 1 is 6.5-7.0 cm in length and 2.0-3.0 cm in width, and the length and width of the oxygen sensing film 2 and the biofilm 3 are both 2.0-3.0 cm.

[0041] Said glucose-glutamic acid standard solution (GGA solution) is to accurately weigh each 0.0750g of analytically pure glucose and glutamic acid, and be settled to 100mL with the above phosphate buffer solution, and the solution obt...

Embodiment 2

[0050] The steps of the automatic activation method for the biochemical oxygen demand microbial sensor sensitive film of the present invention are:

[0051] Add GGA solution with a concentration of 50-200mg / L into the water tank, and immerse the pump in the GGA solution; set the soaking time and aeration time of the sensitive membrane through the dial switch on the control circuit, the soaking time is 5-60min, The aeration time is 1 to 5 minutes; take out 3 to 9 pieces of sensitive film from the refrigerator, put them into the card slot of the activation tank, turn on the power of the activation device, the solenoid valve is in the closed state, the pump starts to rotate, and the GGA solution in the water tank Pump it into the activation tank; the liquid level begins to rise and gradually submerge the sensitive membrane. When the liquid level rises to the high level electrode, the pump stops working, and the sensitive membrane is soaked in the GGA solution; after the soaking ti...

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Abstract

This invention is biochemical oxygen needing microorganism sensor sensitive membranes activation method and its auto active equipment. It relates to a microorganism sensor, it provides a manual active method used to BOD microorganism sensor sensitive membranes and auto activation method and its auto active equipment. When manual method is used, the membranes is soaked to fluorescence signal stable in GGA solution, then GGA solution is discharged and the membranes is aerated to the signal stable. The membranes are soaked with new GGA solution to dynamic balance, soak and aeration is done several times for multiple activations. The equipment is set with basin, pump, control circuit, active slot, liquid level pole and electromagnetic valve. Then auto method is used, the pump is soaked in the GGA solution, the membranes soaking and aeration time is controlled, then the membrane is dropped into the active slot, then the GGA solution in the water slot is taken into the active slot. The membrane is soaked into GGA solution and serration, then soaking and aeration is repeated.

Description

technical field [0001] The invention relates to a microbial sensor, in particular to an activation method and an automatic activation device which can be used for a biochemical oxygen demand (BOD) microbial sensor sensitive film. Background technique [0002] Biochemical oxygen demand (Biochemical Oxygen Demand, BOD) is one of the most commonly used and most important indicators in the process of water quality evaluation. The currently widely used 5-day biochemical oxygen demand standard dilution method is a standard method formulated by the American Public Health Association in 1936. However, the traditional measurement method not only requires time-consuming and complicated operation steps such as 5-day cultivation, but also the accuracy of the measurement has a lot to do with the proficiency and experience of the operator, which cannot realize real-time and online monitoring of environmental water samples. Therefore, research on BOD microbial sensors and related instrume...

Claims

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Application Information

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IPC IPC(8): C12Q1/02G01N33/18
Inventor 陈曦肖来龙辛玲玲叶艺文范朝马越超
Owner XIAMEN UNIV
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