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Inductor of mycorrhizal fungi for cultivating protocorm solid of Tiepi stem of noble dendrobium

A technology of Dendrobium officinale and fungal elicitor, which is applied in the field of bioengineering and can solve problems such as shortage of medicinal resources of Dendrobium officinale.

Inactive Publication Date: 2006-12-06
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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Problems solved by technology

[0008] Another object of the present invention is to increase the active ingredient content of the protocorm of Dendrobium candidum, so that the protocorm of Dendrobium candidum cultured on a large scale can replace the medicinal material of Dendrobium candidum, and solve the problem of shortage of medicinal resources of Dendrobium candidum

Method used

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  • Inductor of mycorrhizal fungi for cultivating protocorm solid of Tiepi stem of noble dendrobium

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Embodiment

[0032] 1. Culture of fungi

[0033] (1) After activating Mycena sp. from the slant test tube strains preserved at low temperature, transfer them to φ90mm PDA plates, cultivate them at 25°C for 24 days, and use a φ9mm hole puncher to separate the colonies in the plates The edge is perforated into bacterial flakes, and small fragments are inserted into the liquid medium for cultivation and fermentation.

[0034] (2) Medium for fungal liquid culture: glucose 2%, KH 2 PO 4 0.3%, MgSO 4 0.15%; natural products: wheat bran 3% (boiled juice), pH5.6.

[0035] (3) Culture conditions: 500 mL of the above-mentioned medium was cultured on a shaking table with a capacity of 250 mL, sterilized at 121° C. for 30 minutes, and inoculated with Mycena sp. Culture conditions: shaker speed 120 rpm, temperature: 25°C, cultured in dark for 21 days and harvested.

[0036] 2. Preparation of Fungal Elicitors

[0037] After the fungus of the genus Mycena (Mycena sp.) was fermented and cultured, it...

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Abstract

This invention discloses a technique for applying MF24 (Mycena sp.) in the culture of protocorm in Dendrobium candidum. The technique comprises: (1) culturing protocorm in Dendrobium candidum and MF24 (Mycena sp.), respectively; (2) manufacturing MF24 (Mycena sp.) into inducer, and adding into protocorm in Dendrobium candidum for co-culture. The cultured protocorm in Dendrobium candidum has higher yield and contents of effective components, and can be used to replace Dendrobium candidum as a pharmaceutical material. The method is simple, and does not need addition of any hormone.

Description

technical field [0001] The invention belongs to the technical field of bioengineering. Specifically, it relates to the application of a fungal elicitor in the solid culture of protocorms of Dendrobium candidum and a practical technique for increasing the content of active ingredients in the protocorms of Dendrobium candidum. Background technique [0002] Dendrobium is a traditional precious Chinese herbal medicine, first recorded in "Shen Nong's Materia Medica", listed as top grade. Dendrobium is a plant of the genus Dendrobium in the family Orchidaceae. The whole herb is used as medicine. It is sweet in taste and slightly cold in nature. It has the effects of nourishing the stomach and promoting body fluid, nourishing yin and removing heat. Dendrobium candidum Wall.ex Ldl. is one of the five species of Dendrobium recorded in Chinese Pharmacopoeia. It has been considered as a treasure for nourishing yin and tonifying since ancient times. "Compendium of Materia Medica" reco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04C12N1/14
Inventor 郭顺星陈晓梅王春兰孟志霞肖培根
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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