Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Triptolide purification method

A purification method, the technology of Tripterygium wilfordii, which is applied in the field of purification of triptolide, can solve the problems of high cost, unfavorable industrial production, and low efficiency, and achieve the effects of short preparation time, reduced volume, and simple operation

Active Publication Date: 2006-12-13
CHINESE MEDICINE RES INST SHANGHAI
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If extracts of this quality are directly applied to column chromatography to separate A, the efficiency of its preparation work must be low, and the cost is also quite expensive, which is not conducive to possible industrial production in the future

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Triptolide purification method
  • Triptolide purification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Get 2kg of Tripterygium wilfordii extract (triptolide alcohol content 1%), 12kg of silica gel, add 15kg of chloroform, fully stir under 30 ℃ of heat preservation conditions, extract is fully dissolved, then filter, filter cake is washed several times with chloroform, chloroform The total consumption is 30kg. Combine the chloroform liquid, shrink to dry, obtain purifying Tripterygium wilfordii extract 0.9kg, its triptolide content is 2.2% (HPLC method) see figure 1 , figure 2 It is the reference substance of triptolide.

Embodiment 2

[0023] Get 1 kg of Tripterygium wilfordii extract (triptolide content 1%), 5 kg of neutral aluminum oxide, add 10 kg of ethyl acetate, fully stir at 40 ° C under heat preservation conditions, dissolve the extract, then filter, and use the remaining 5 kg for the filter cake. After washing with ethyl acetate several times, the ethyl acetate filtrate and washings were combined, concentrated in vacuo, and dried to obtain 0.5 kg of purified extract with a triptolide alcohol content of 2.0%.

Embodiment 3

[0025] Get 1kg of Tripterygium wilfordii extract (triptolide alcohol content 1%), diatomaceous earth 10kg, with 15kg acetone-cyclohexane (1:1), fully stir at 35 ℃, dissolve extract, then filter, filter cake Wash several times with 10 kg of the above-mentioned solvent, combine the filtrate and washing liquid, concentrate in vacuo, and dry to obtain 0.6 kg of purified extract, whose triptolide content is 1.7%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates the purity method of triptolide alcohol, comprising the following steps: using tripterygium wilfordii as raw material, adding adsorbent and dispersion dissolvent, adsorbing, filtering, thickening, drying, and getting tripterygium wilfordii extract concrete. The method has the advantages of simple operation, short time and high triptolide alcohol transition rate.

Description

technical field [0001] The invention relates to the technical field of preparation of traditional Chinese medicines, in particular to a method for purifying triptolide. Background technique [0002] Triptolide (triptolide), i.e. triptolide, is one of the main components of triptolide, it is the main active ingredient of immunosuppression in tripterygium preparations, and it is also a toxic component. Its content in current traditional Chinese medicine preparations There are strict controls. [0003] Since the effective dose of triptolide is very close to the toxic dose, the possibility of developing new drugs with a single component is basically denied. However, as an effective lead drug, pharmacists at home and abroad have been continuously screening triptolide derivatives for more than 20 years, and made a lot of gratifying progress. [0004] For the research and development of potential new drugs of triptolide derivatives, the preparation and supply of triptolide with t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/22C07D303/00C07D307/00
Inventor 张国明华菊根袁华梁
Owner CHINESE MEDICINE RES INST SHANGHAI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com