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Process of producing live microbial composition EM prepn

A microbial composition and production method technology, applied in the direction of biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of affecting the growth of EM live bacteria, affecting product quality, and expensive raw materials, so as to enhance the disease resistance of livestock and poultry, Enhances immunity and removes feces odor

Active Publication Date: 2007-01-03
中健生物科技(深圳)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This culture medium formula, the raw material that adopts is more expensive, the cost is high, relatively speaking, the nutrient is not comprehensive, such as trace elements such as sulfur, phosphorus, potassium, magnesium, etc. are not added, which will inevitably affect the growth of EM live bacteria, thereby affecting to product quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Take rice: wash water = 1:1 (weight ratio) of rice washing water 1000g, use Na 2 CO 3 After the solution was adjusted to pH 7.0, 0.1 g (0.01%) of neutral protease was added, stirred evenly, heated to 40° C. for 1 hour, and stirred once every 10 minutes. Then add NaCl 1g (0.1%), waste molasses 20g (2%), KH 2 PO 4 1g (0.1%), MgSO 4 0.2g (0.02%), 1g (0.1%) of yeast extract, mix and stir evenly, boil for 5 minutes, wait to cool to 35°C, inoculate 150g (15%) of live EM bacteria, mix well and place in a plastic tank to seal, ferment temperature 24~28℃, the fermentation time is 96 hours. PH is 3.5, the number of bacteria reaches 3.6×10 9 pcs / ml, the smell is sour and sweet.

Embodiment 2

[0019] Take rice: washing water = 1:2 (weight ratio) 500kg of first rice washing water, add appropriate amount of Na 2 CO 3 After the solution was adjusted to pH 7.0, 50 g (0.01%) of neutral protease was added, stirred evenly, heated at 45° C. for 1 hour, and stirred once every 10 minutes. Then add NaCl 1kg (0.2%), waste molasses 10kg (2%), KH 2 PO 4 1kg (0.2%), MgSO 4 0.075kg (0.015%), yeast extract 1.0kg (0.2%), mix and stir evenly, heat at 100°C for 5 minutes, cool to 35-40°C, inoculate 50kg (10%) of live EM bacteria, mix evenly, and ferment at It is 33 ℃, and the fermentation time is 85 hours. The pH is 3.5, and the number of bacteria reaches 3.7×10 9 pcs / ml, the smell is sour and sweet.

Embodiment 3

[0021] Take rice: washing water = 1: 2.5 (weight ratio) 500kg of first rice washing water, add appropriate amount of Na 2 CO 3 After the solution was adjusted to pH 7.0, 20 g (0.004%) of neutral protease was added, stirred evenly, heated at 45° C. for 1 hour, and stirred once every 10 minutes. Then add NaCl 1.5kg (0.3%), starch saccharification slurry 45kg (9%), KH 2 PO 4 1.5kg (0.3%), MgSO 4 0.075kg (0.015%), 1kg (0.2%) of yeast extract, mix and stir evenly, heat at 100°C for 5 minutes, wait to cool to 35-40°C, inoculate 50kg (10%) of live EM bacteria, mix evenly, and ferment at 35°C, the fermentation time is 85 hours. The pH is 3.5, and the number of bacteria reaches 3.7×10 9 pcs / ml, the smell is sour and sweet.

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PUM

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Abstract

The live microbial composition EM preparation is produced with rice washing water and through regulating pH value, treating with neutral proteinase, adding certain amount of one of waste molasses, red sugar and starch saccarifying slurry, phosphate, table salt, sulfate and yeast paste, mixing homogeneously, heating at 100 deg.c for 5 min, cooling, inoculating live EM bacterial liquid in 10-20 %, and fermentation to culture. The present invention has simple production process suitable for industrial production and low cost, and can produce high quality live microbial composition EM preparation with high live bacteria content for use in farm production, livestock breeding, food processing, environment protection and other fields.

Description

technical field [0001] The invention relates to a production method of a microbial composition EM live bacteria agent. Background technique [0002] EM is the English abbreviation of effective microorganisms (efective microorganisms), which is a relatively common microecological preparation at present. It is mainly composed of 10 genera and 80 kinds of microorganisms such as photosynthetic bacteria, lactic acid bacteria, actinomycetes, and fermentative filamentous bacteria. It is a special bacterial group with both anaerobic and aerobic properties. It is first of all a new type of compound microbial living bacteria preparation successfully researched by Professor Higa Teruo of the University of Ryukyu in Japan. EM does not contain any harmful chemical substances, has no toxic side effects, no residues and secondary pollution, and does not produce anti-drugs. Since it came out in the 1980s, it has been promoted and used in more than 60 countries and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00
Inventor 周马林张学荣
Owner 中健生物科技(深圳)有限公司
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