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Free cobalt quantitative determination method of serum blood deficiency decorated albumin

A technique for quantitative determination of albumin, applied in the field of quantitative determination of free cobalt of serum ischemia-modified albumin, to achieve the effect of avoiding instability

Inactive Publication Date: 2007-01-31
李海燕
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Problems solved by technology

[0009] The purpose of the present invention is to express the degree of binding between serum and cobalt with free cobalt content by detecting the remaining cobalt content in the reaction system, expressing the decline of the binding force between ischemia-modified albumin and cobalt, and fundamentally solving the problem of ischemia-modified albumin Quantify the problem and report the test result as free cobalt concentration

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  • Free cobalt quantitative determination method of serum blood deficiency decorated albumin

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Embodiment Construction

[0017] The present invention uses dithioerythritol (DTE) as a chromogenic reagent for detecting free cobalt in the reaction system as an example to describe the specific implementation. The test tube used is 180 μl of serum, add 120 μl of 2.48 mmol / L cobalt chloride solution, add 1600 ul of Tris-HCl buffer solution with pH 7.8, mix well and put it in a water bath at 37°C for 8 minutes, add 200 ul of 5 mmol / L DTE, mix Put evenly and incubate at 37°C for 2 minutes, and measure the free cobalt concentration by colorimetric at a wavelength of 480nm under a UV-2800 ultraviolet-visible spectrophotometer. The specific test plan is shown in Table 1:

[0018] Assay tube

standard tube

Assay control tube

standard control tube

Serum (μl)

180

--

180

--

Standard solution (μl)

--

180

--

180

[0019] Cobalt reagent (μl)

120

120

120

120

Buffer (μl)

1600

...

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Abstract

The invention relates to a free cobalt quantitative determination method for serum ischemia decorating albumin. It uses the weakening of compound ability of ischemia decorating albumin, testing the dissociated cobalt in reacting system by chemical agent to gain the alteration of absorbance to represent the alteration of the content of dissociated cobalt, and indirectly expressing the thickness of ischemia decorating albumin. According to the testing result, the relationship curve of thickness index and absorbance would be made, and determining the linear relation between cobalt thickness and absorbance is C measured= A (measured- measured comparison tube) / A (standard- standard comparison tube)* C standard. The invention could avoid instability caused by the difference between devices or agents.

Description

technical field [0001] The invention relates to a method for detecting ischemic modified albumin in human serum. In this method, the combination of ischemia-modified albumin and exogenous Co in the serum of patients with acute coronary syndrome (ACS) 2+ Due to the weakened binding ability, a certain concentration of cobalt solution is added to the serum to fully combine with the albumin in the serum, and then a reagent is added to detect the free cobalt in the reaction system, and the change of the free cobalt content is indicated by the change of absorbance, which indirectly indicates ischemia Modify the concentration of albumin. Background technique [0002] Human serum albumin (HSA) is a circulating protein whose amino-terminal sequence is unique to humans and can bind to metal cobalt, copper, and nickel ions. Compared with other species of animal albumin, the metal-binding site of HSA is easier Degraded by biochemical factors. Under the action of oxygen free radicals,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/31
Inventor 陈宝荣方虹杨振华
Owner 李海燕
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