Method for preparing red flower extract containing total red flower uranidin

A technology of safflower extract and yellow pigment, which is applied in the direction of medical preparations containing active ingredients, drug combinations, pharmaceutical formulas, etc., can solve the problems of poor stability and safety of preparations, insufficient refining degree, unclear composition and amount of impurities and other issues, to achieve the effect of improving stability and ensuring the safety of preparations

Active Publication Date: 2007-03-28
BEIJING SUNHO PHARMA
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Problems solved by technology

The above two patents use polar solvent extraction, the extract is simply treated and then separated and purified with an adsorbent to absorb the active ingredients, and the extract is obtained after drying. This method improves the quality of the safflower extract to a certain extent, but due to The composition of the active ingredients and the amount of impurities in the extract prepared by this method are not clear, resulting in poor stability and safety of the preparation developed by it, especially not suitable for injections. With the clinical application of traditional Chinese medicine injections There are many reports of adverse reactions caused by traditional Chinese medicine injections [Mo Binbin et al., Statistical analysis of literature on adverse reactions of traditional Chinese medicine injections, Zhongnan Pharmacy, 2003, 1(3): 184-185], most of which are due to the use of traditional It is caused by the extraction process and the degree of refinement is insufficient, so its drug safety is a concern and concern in the industry

Method used

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  • Method for preparing red flower extract containing total red flower uranidin
  • Method for preparing red flower extract containing total red flower uranidin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1 Preparation of safflower extract containing total safflower yellow by water extraction

[0065] Take 2000g of safflower medicinal material, add 10 times of water (the weight of the medicinal material), stir and extract twice at 70°C for 1 hour each time, filter, combine the secondary extracts, and cool to room temperature. According to the ratio of the amount of sample on the column to the amount of resin (1:10 / W:V), pass through HP-20 macroporous adsorption resin column chromatography, the diameter-to-height ratio of the column is 1:7, and use water at 1-2ml / cm 2 / min flow rate elution 1 column bed volume discarded, followed by 25% ethanol 1-2ml / cm 2The flow rate per minute is 4 column bed volumes, the eluted part of 30% ethanol is the total safflower yellow part, the solvent is recovered under reduced pressure at 70 ° C, and the concentrated solution with a specific gravity of about 1.05 is passed through the ceramic with a molecular weight cut-off of 8000. ...

Embodiment 2

[0067] Example 2 Preparation of safflower extract containing total safflower yellow by water extraction

[0068] Take 2000g of safflower medicinal material, add 8 times of water (medicinal material weight), stir and extract twice at 75°C for 1 hour each time, filter, combine the secondary extracts, and cool to room temperature. According to the ratio of the amount of sample on the column to the amount of resin (1:8 / W:V), add HPD 600 macroporous adsorption resin column chromatography, the diameter-to-height ratio of the column is 1:8, and use water to 2 / min flow rate elution 1 column bed volume discarded, followed by 40% ethanol 1-2ml / cm 2 The flow rate per minute is 4 column bed volumes, the 40% ethanol elution part is the total safflower yellow part, the solvent is recovered under reduced pressure at 70 ° C, and the specific gravity is about 1.02. The membrane is subjected to ultrafiltration, and the filtrate is collected and dried to obtain the total safflower yellow of th...

Embodiment 3

[0070] Example 3 Preparation of safflower extract containing total safflower yellow pigment by dilute alcohol extraction

[0071] Get 2000g of safflower medicinal material, add 10 times of 30% ethanol (medicinal material weight), reflux extraction 2 times, each time for 1 hour, filter, merge the secondary extract, reclaim the extract to no alcohol, according to the amount of sample on the column and Resin amount (1:10 / W:V) ratio, add HP-20 macroporous adsorption resin column chromatography, the diameter-to-height ratio of the column is 1:10, use water at 1-2ml / cm 2 / min flow rate elution 1 column bed volume discarded, followed by 30% ethanol 1-2ml / cm 2 The flow rate per minute is 4 column bed volumes, the part eluted with 35% ethanol is the safflower yellow part, and the solvent is recovered under reduced pressure at 70°C to a concentrated solution with a specific gravity of about 1.05, and the above solution is passed through an organic membrane with a molecular weight cut-of...

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Abstract

The present invention relates to a preparation method of carthamus extract. Said method includes the following steps: soaking carthamus flower in warm water to obtain extract A; concentrating the extract A to obtain concerrtrate, separating said concentrate by means of macroporous adsorbing resin, then successively using water and alcohol to make elution, collecting eluted portion by using alcohol, reeovering eluent until the alcohol content is below 25% so as to obtain eluent B; using ceramic membrane to make ultrafiltration so as to obtain ultrafiltrate C; decompressing ultrafiltrate, recovering solvent to obtain heavy paste, drying so as to obtain the invented product. Said extract is characterized by that total carthamus yellow content is greater than 80%, hydroxyl carthamus yellow A content is greater than 20% and kaempferol-3-0-rutinoside content is greater than 0.6%.

Description

technical field [0001] The present invention relates to a preparation method of safflower extract, specifically, the safflower extract containing total safflower yellow pigment and its preparation method, as well as the pharmaceutical composition containing the extract and its application. Background technique [0002] The traditional Chinese medicine safflower is the dried flower of the Compositae plant Carthamus tinctorius L. It is a traditional medicine for promoting blood circulation and removing blood stasis. It has a good curative effect on many blood circulation disorders and diseases. At the same time, the total safflower yellow is widely used as a food coloring because of its good coloring ability and strong stability. Since the 1970s, the research on the chemical components of the traditional Chinese medicine safflower has been very in-depth, and its active ingredients have been determined to be water-soluble total safflower yellow, and its main effective monomer c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/286A61K31/352A61P9/10
Inventor 张树祥刘文辉
Owner BEIJING SUNHO PHARMA
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