Method of extracting undenatured natural collagen from tanning waste leather offcut

A technology of natural collagen and scraps, applied in the field of extracting undenatured natural collagen, can solve the problems of low added value and no biological activity, and achieve the effect of unique biocompatibility, low biodegradability and broad application prospects

Inactive Publication Date: 2007-04-18
柳州市宏升胶原蛋白肠衣有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] To sum up, the products extracted from the leather scrap waste produced in tanning are all hydrolyzed collagen, which has no biological activity and low added value

Method used

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  • Method of extracting undenatured natural collagen from tanning waste leather offcut
  • Method of extracting undenatured natural collagen from tanning waste leather offcut
  • Method of extracting undenatured natural collagen from tanning waste leather offcut

Examples

Experimental program
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Effect test

Embodiment 1

[0029]Embodiment 1: get the tanned skin dregs of pig slices, mince them, wash 3 times with warm water at 30-35° C., take 100 g of debris, add 2 g of fatty acid polyoxyethylene ethers, and 3 g of alkylphenol polyoxyethylene ethers. The solution was soaked for 3 hours at a temperature of 32°C. After filtration, 10 g of isopropanol was added and shaken at room temperature for 24 hours, and then filtered. Adjust the pH to 2 with acid, add 2 g of pepsin, and stir at a temperature of 4-6° C. for 24 hours. Remove the filter residue by centrifugation, add 0.7M NaCl to the supernatant for salting out, dissolve the precipitate with 0.5M acetic acid, repeat the salting out once, and then process through membrane dialysis to obtain a 5% undenatured natural pigskin collagen solution of about 600g.

Embodiment 2

[0030] Embodiment 2: get the tanned skin dregs of pig slices, mince them, wash 3 times with warm water at 30-35° C., take 100 g of debris, and put them into a mixture made of 10 g of fatty acid polyoxyethylene ether and 5 g of alkylphenol polyoxyethylene ether. soaked in the solution for 24 hours at a temperature of 34° C., after filtering, add 50 g of acetone and shake at room temperature for 48 hours, and filter. Adjust the pH to 4 with acid, add 5 g of 537 protease, and stir for 48 hours at a temperature of 4-6°C. Remove the filter residue by centrifugation, add 2.1M NaCl to the supernatant for salting out, dissolve the precipitate with 1.0M acetic acid, repeat the salting out for 3 times, and then process through membrane dialysis to obtain an undenatured natural pigskin collagen solution with a concentration of about 8%. 600g.

Embodiment 3

[0031] Embodiment 3: get the offcuts of beef slices ash-alkali skin, mince, get 100g, adjust pH=8 with 2M NaOH solution to make the skin swell for 24 hours, use concentration of 30g / L solution pH=8 H 2 o 2 Bleach for 3 hours, then use 200g of 0.5M EDTA decalcification solution to decalcify for 24 hours, fully wash with tap water, filter to obtain sediment, adjust pH=2.5 with acid, add 2g of pepsin, and stir for 24 hours at a temperature of 4-6°C. The filter residue was removed by centrifugation, 0.7M NaCl was added to the supernatant for salting out, and the precipitate was dissolved with 0.5M acetic acid to obtain about 600 g of undenatured natural collagen solution of bovine hide with a concentration of 5%.

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Abstract

This invention relates to an extraction method of un-denatued natural collagen from tanning scrap material. The feature is that 100 weight amount splitting glaubered pigskin scrap material is washed with 2-5 amount water for 3-5 times at 30-35DEG C and is soaked with 5-15 amount for 3-6 hours. After repeating twice, filtering, and then treating it with 10-50 amount organic solvent for 12-24 hours, and then filtering for sediment. Or 100 weight amount splitting limed buffalo-hide scrap material is swelled for 24-48 hours with pH adjusted to 8-12, and bleached with H2O2 with pH=8-12 and concentration 15-60g / L, and then Decalcified, washed, filtered for sediment. Either above-mentioned 100 amounts of sediment is added into protease 0.5-6 amount and adjusted with acid to pH=2-4, and stirred for 1-3 days at 4-6DEG C, and then collagen solution is obtained by centrifugal separation, and then salting out is conducted with 0.5-3MNaC. The sediment is treated with 0.05-2M acid for twice after centrifugal separation, at last, 5-8% un-denatued pigskin or buffalo-hide collagen protein liquid is produced by membrane dialysis.

Description

technical field [0001] The invention relates to a method for extracting undenatured natural collagen from tanning waste leather leftovers, belonging to the field of high value-added transformation of tanning solid waste. Background technique [0002] According to incomplete statistics, my country processes 170 million pieces of leather such as pigs and cattle every year, and produces about 700,000 tons of scrap waste (without chromium). Among them, it is mainly raw leather trimming scraps, scraps of gray leather or scraps produced by tanned leather. These solid wastes are one of the important factors causing serious pollution in the leather industry. Since their main component is collagen, using leather leftovers to extract high-quality collagen and develop it into high-value-added products can not only provide a way for the leather industry to solve solid waste pollution, but more importantly, animal matter resources The comprehensive development and high-value utilizatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09H3/00
Inventor 李国英赵帅李东李洋
Owner 柳州市宏升胶原蛋白肠衣有限公司
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