Wave-guide coupling surface plasma resonance biosensor

Abstract

A waveguide coupling surface plasma resonance biosensor is disclosed that includes: a grating layer, a waveguide layer, a plasma resonance layer and a ligand layer. The grating layer which has a periodical first grating structure is made of translucent material, the waveguide layer is formed on the first grating structure, and the refractive index of the waveguide layer is bigger than the refractive index of the grating layer. The plasma resonance is formed on the waveguide layer which can generate plasma resonance by means of light wave excitation. The ligand layer is formed on the plasma resonance layer, and the ligand layer can generate combination reaction with the detecting substance. The analytical platform of molecular interaction can be built using the invention, and a detecting method without marking can detect the physical quantities of molecular interaction of chemical material, reaction rate and molecular dynamics.

Description

technical field

[0001] The present invention relates to a biosensor, in particular to a waveguide-coupled surface plasmon resonance biosensor that uses gratings and metal materials to establish an interaction analysis platform for biomolecules that does not require any labeling procedures to measure the physical quantities of intermolecular interactions in real time. . Background technique

[0002] In biomolecular interaction analysis (BIA), the development and application of biochips is one of the key technologies for modern biotechnology to move from genomics to proteomics. Biochips are useful tools for simultaneously detecting a large number of gene expressions or detecting trace amounts of biomolecular information. Today's detection is still dominated by fluorescence detection. However, the fluorescent system involves cumbersome fluorescent labeling, the difficulty of some molecular labeling, the inevitable fluorescence decay, and the difficulty of providing real-time k...

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