Plant seed transformation technology
A plant seed and seed technology, applied in the biological field, can solve the problems of difficult regeneration system, limitation, genetic background difference, etc., and achieve the effect of improving transformation efficiency and great application value
Inactive Publication Date: 2010-11-10
TIANJIN UNIV OF SCI & TECH
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Problems solved by technology
However, due to factors such as difficult regeneration systems and low conversion rates due to differences in genetic backgrounds, they are still in the process of continuous improvement.
The use of physical means (such as ultrasonic wave, negative pressure method, pulse electrophoresis, heavy ion irradiation and gamma ray irradiation, etc.) also has major problems such as limited use of materials and low conversion rate.
Method used
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The invention discloses a switching technique of multiple-typed plant, which is characterized by the following: switching plant seed irradiated by gamma-ray (dose: 0.01-10Gy; dose rate: 0.1-2Gy / h); germinating in the selective gene; testing; sieving instantaneous expressive seed of GUS gene; adopting positive seed as explant; obtaining regenerative plant on the culture medium; planting positive seed in the flowerpot or field directly for 3-5d; dripping 50-200 mul relative antibiotics with density at 50-250mg / l on the stem top until the flower bud forms; collecting seed; identifying the gene;planting positive material.
Description
Plant seed transformation method technical field The invention patent belongs to the field of biotechnology. Background technique Exogenous DNA enters the plant genome, and currently there are mainly technologies such as Agrobacterium-mediated callus and gene gun method. However, due to factors such as difficult regeneration systems and low conversion rates due to differences in genetic backgrounds, they are still in the process of continuous improvement. The use of physical means (such as ultrasonic wave, negative pressure method, pulse electrophoresis, heavy ion irradiation and γ-ray irradiation, etc.) also has major problems such as limited materials and low conversion rate. Wherein the gamma ray irradiation similar to the present invention, because the irradiation material is callus, problems such as regeneration system, irradiation period and pollution have to be taken into consideration, and the actual operation is greatly restricted. Therefore, in the face of the ...
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IPC IPC(8): C12N15/82A01H4/00
Inventor 李兴林舒庆尧路福平夏英武吴殿星高年发张健崔海瑞傅俊杰
Owner TIANJIN UNIV OF SCI & TECH