Agents for preventing and treating HIV and other viral infections
a technology for viral infections and agents, applied in the field of agents for preventing and treating hiv and other viral infections, can solve the problems of high frequency of infections, inability to cure the disease, physical and mental stress resulting from the permanent need to take drugs, etc., and achieve the effect of reducing the infection efficiency of hiv and preventing the development of hiv-associated bacteria
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exemplary embodiment 1
ic Inhibitor D3 for Reducing the SEVI- or Aβ-Mediated Viral Infection
[0109]So as to analyze the effect of the amyloidogenic inhibitor D3 as a potential therapeutic agent for reducing amyloid peptide-promoted retroviral propagation, adherently growing TZM-bl cells were infected with HIV-1. These cells express the HIV-1 receptors CD4, CXCR4 and CCR5, which is why they are permissive both for T-tropic and M-tropic HIV isolates. Furthermore, they express the reporter genes β-galactosidase and luciferase under the control of the HIV-1 LTR promoter, the expression of which can thus be induced by an HIV-1 infection and consequently demonstrated. The TZM-bl indicator cell line thus allows a quantitative analysis of HIV infection efficiency.
[0110]TZM-bl cells were infected with equal amounts of HIV-1, which had previously been incubated with different concentrations of Aβ1-42 fibrils, which, in turn, had been treated with two concentrations of the peptide D3 or PBS as solvent control. D3 was...
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