Method for diagnosing selected adenocarcinomas

a technology for adenocarcinomas and adenocarcinomas, which is applied in the field of diagnostic methods for selected adenocarcinomas, can solve the problems of false positive, increased blood loss in the gastro-intestinal tract, and false positiv

Inactive Publication Date: 2003-02-13
TABIBZADEH SIAMAK
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Problems solved by technology

In addition, there are other factors which could result in a false positive result for this test.
For example, it has been found that aspirin and other non-inflammatory analgesics have been known to cause irritation in the stomach and increased gastro-intestinal tract blood loss, thereby producing in a false positive result.
The patient's ingestion of rare beef and fruits and vegetables which contain catalases and peroxidases within 24 hours of administering the test may also cause a false positive result.
However, soon after this test became available to health professionals, it was observed that this test was simply too insensitive to recognize numerous types of colon cancers.
Even in this role though, it has met with only limited success.
However, researchers have also acknowledged that this screening method contains inherent limitations.
Moreover, general patient discomfort while undergoing this screening is believed to be one of the obstacles in providing mass screening for the general population.
Finally, health professionals acknowledge that there is a very slight risk of perforating a patient's colon while undergoing the procedure.
However, there is only a 20 to 25% chance of

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  • Method for diagnosing selected adenocarcinomas
  • Method for diagnosing selected adenocarcinomas
  • Method for diagnosing selected adenocarcinomas

Examples

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example 1

Detection of Mucinous Adenocarcinomas of the Colon

[0040] In FIG. 2, 20 mg total RNA from a normal late secretory endometrium which served as the positive control (lane 1) as well as mucinous adenocarcinomas of colon (lanes 2, 4, 6) non-mucinous adenocarcinomas of colon (lanes 3, 10, and 12) and adjacent normal colon (lanes 3, 7, 9, 11 and 13) was subjected to the Northern blot analysis using the entire placental-derived ebaf cDNA as the probe (upper panel). Here, the expression of a 2.1 kb ebaf mRNA was detected in seven of the eleven cases of adenocarcinomas of the colon. The histological evaluation of the positive cases revealed them to have a mucinous differentiation.

example 2

Detection of adenocarcinomas of the Testis

[0041] FIG. 3 is a Northern blot displaying the results of an examination of five cases of testicular cancer for the expression of ebaf gene. 20 mg total RNA from a normal menstrual endometrium which served as the positive control (lane 1) and each tumor tissue (lane 2: teratoma-embryonal cell carcinoma, lane 3: mixed germ cell tumor containing embryonal carcinoma, lanes 4-6; seminoma) were subjected to the Northern blot analysis using the entire placental-derived ebaf CDNA as the probe (upper panel). The blot was exposed for long duration to detect ebaf mRNA in the neoplastic tissues. This resulted in the overexposure of the ebaf mRNAs detected in the endometrium.

[0042] The results shown in FIG. 3 indicate that a 2.5 kb ebaf mRNA is detected in the tumors containing embryonal carcinoma. The 2.1 kb mRNA is also detected in two out of three cases of seminoma. The integrity of RNA and equal loading was verified by staining the 18S and 28S ribo...

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Abstract

A method for the early diagnosing of selected adenocarcinomas in a human comprising the steps of removing a bodily sample from the human, and assaying the bodily sample for elevated expression of a specific gene. The gene being assayed for in the bodily sample is the TGFB-4 gene (hereinafter referred to as the endometrial bleeding associated factor (ebaf) gene. The bodily sample can be tissue from a specific organ in the body, or a blood sample. Increased levels of ebaf in the sample relative to basal levels may be indicative of a mucinous adenocarcinoma of the colon or ovaries, or an adenocarcinoma of the testis.

Description

CROSS REFERENCE[0001] This application claims the benefit of U.S. Provisional Application Ser. No. 60 / 025,800, entitled "Method for the Early diagnosis of Colon Cancer, Abnormal Uterine Bleeding, or Pregnancy", filed Aug. 27, 1996.[0003] A method for diagnosis of human bodily conditions, and more particularly, a method for diagnosing selected mucinous adenocarcinoma of the colon, or ovaries, or an adenocarcinoma of the testis.BACKGROUND OF INVENTION[0004] Early diagnosis of particular pathological conditions of the human body can provide patients with adequate time to make well informed decisions regarding the treatment of their pathological condition, as well as prepare for the potential incapacitation of the patient. One such pathological condition is colon cancer. The American Chemical Society has reported that colon cancer is the second most common cause of cancer in the United States (Fleischer, D. et al. Detection and Survival of Colorectal Cancer (1989) JAM 261 (4) :580. It h...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/574
CPCC12Q1/6886C12Q2600/158G01N33/57407G01N33/57419G01N33/57442G01N33/57449G01N2333/495
Inventor TABIBZADEH, SIAMAK
Owner TABIBZADEH SIAMAK
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