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Isolated human metalloprotease proteins, nucleic acid molecules encoding human protease proteins, and uses thereof

Inactive Publication Date: 2005-03-17
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0036]FIG. 2 provides the predicted amino acid sequence of the protease of the present invention. (SEQ ID NO:2) In addition structure and functional information such as pr

Problems solved by technology

Thus, complex and highly-regulated mechanisms have been evolved to accomplish this degradation.
For example, oxidative stress has been shown to damage a variety of proteins and cause them to be rapidly degraded.
Trypsin-I in complex with alpha-1-antitrypsin, also called alpha-1-antiprotease, has been found to occur in serum of patients with pancreatitis (Borgstrom A and Ohlsson K, Scand J Clin Lab Invest 1984; 44: 381-6) but determination of this complex has not been found useful for differentiation between pancreatic and other gastrointestinal diseases (Borgstrom et al., Scand J Clin Lab Invest 1989; 49:757-62).
Chronic pancreatitis often results in permanent damage.
In addition, any internal or external blockage of pancreatic ducts can also cause an accumulation of pancreatic juices in the pancreas resulting cellular damage.
So far, success in obtaining amino acid sequence information from these fungal neutral metalloproteases has been limited.
However, to date, no npI fungal metalloprotease has been cloned or sequenced.

Method used

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  • Isolated human metalloprotease proteins, nucleic acid molecules encoding human protease proteins, and uses thereof
  • Isolated human metalloprotease proteins, nucleic acid molecules encoding human protease proteins, and uses thereof
  • Isolated human metalloprotease proteins, nucleic acid molecules encoding human protease proteins, and uses thereof

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specific embodiments

[0041] Peptide Molecules

[0042] The present invention provides nucleic acid sequences that encode protein molecules that have been identified as being members of the protease family of proteins and are related to the metalloprotease subfamily (protein sequences are provided in FIG. 2, transcript / cDNA sequences are provided in FIG. 1 and genomic sequences are provided in, FIG. 3). The peptide sequences provided in FIG. 2, as well as the obvious variants described herein, particularly allelic variants as identified herein and using the information in FIG. 3, will be referred herein as the protease peptides of the present invention, protease peptides, or peptides / proteins of the present invention.

[0043] The present invention provides isolated peptide and protein molecules that consist of, consist essentially of, or comprise the amino acid sequences of the protease peptides disclosed in the FIG. 2, (encoded by the nucleic acid molecule shown in FIG. 1, transcript / cDNA or FIG. 3, genomi...

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Abstract

The present invention provides amino acid sequences of peptides that are encoded by genes within the human genome, the protease peptides of the present invention. The present invention specifically provides isolated peptide and nucleic acid molecules, methods of identifying orthologs and paralogs of the protease peptides, and methods of identifying modulators of the protease peptides.

Description

FIELD OF THE INVENTION [0001] The present invention is in the field of protease proteins that are related to the metalloprotease subfamily, recombinant DNA molecules, and protein production. The present invention specifically provides novel peptides and proteins that effect protein cleavage / processing / turnover and nucleic acid molecules encoding such peptide and protein molecules, all of which are useful in the development of human therapeutics and diagnostic compositions and methods. BACKGROUND OF THE INVENTION [0002] The proteases may be categorized into families by the different amino acid sequences (generally between 2 and 10 residues) located on either side of the cleavage site of the protease. [0003] The proper functioning of the cell requires careful control of the levels of important structural proteins, enzymes, and regulatory proteins. One of the ways that cells can reduce the steady state level of a particular protein is by proteolytic degradation. Further, one of the way...

Claims

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Application Information

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IPC IPC(8): C12N1/21C12N9/64C12N9/70C12N15/55
CPCC12N9/6489A01K2217/05
Inventor MERKULOV, GENNADYYE, JANEDI FRANCESCO, VALENTINABEASLEY, ELLEN
Owner APPLERA