Systematic evaluation of sequence and activity relationships using site evaluation libraries for engineering multiple properties
Patent Information
- Authority / Receiving Office
- US · United States
- Patent Type
- Applications(United States)
- Current Assignee / Owner
- DANISCO US INC
- Publication Date
- 2008-01-03
- Estimated Expiration
- Not applicable · inactive patent
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Abstract
Description
[0001] The present application claims priority to pending U.S. Provisional Patent Application Ser. No. 60 / 816,202, filed Jun. 23, 2006, and pending U.S. Provisional Patent Application Ser. No. 60 / 933,312, filed Jun. 6, 2007.FIELD OF THE INVENTION
[0002] The present invention provides methods for protein engineering. Specifically, the invention provides methods utilizing site evaluation libraries. BACKGROUND OF THE INVENTION
[0003] Various protein engineering methods are known to those in the art. In general, proteins are modified in order to obtain desired protein properties. In most methods, the nucleotide sequence of a cloned gene encoding a protein is mutated and the modified gene is expressed to produce mutants, which are screened for activities of interest. Often, the mutant properties are compared with the properties of wild-type protein.
[0004] Historically, the protein design process has been approached as equivalent to the problem of finding in all of protein space the one ...