Penicillin g biosensor, systems comprising the same, and measurement using the systems

Abstract

A penicillin G biosensor, systems comprising the same, and measurement using the systems. The penicillin G biosensor has an extended gate field effect transistor (EGFET) structure and comprises a metal oxide semiconductor field effect transistor (MOSFET) on a semiconductor substrate, a sensing unit comprising a substrate, a tin oxide film on the substrate, and a penicillin G acylase film immobilized on the tin oxide film, and a conductive wire connecting the MOSFET and the sensing unit.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application is a Divisional of co-pending application Ser. No. 11 / 024,669, filed on Dec. 30, 2004, and for which priority is claimed under 35 U.S.C. § 120; and this application claims priority of Application No. 092137624 filed in Taiwan on Dec. 31, 2003 under 35 U.S.C. § 119; the entire contents of all of which are hereby incorporated by reference.BACKGROUND OF THE INVENTION[0002]1. Field of the Invention[0003]The invention relates to a biosensor, and more specifically to a biosensor measuring penicillin G concentration and systems comprising the same.[0004]2. Brief Discussion of the Related Art[0005]Penicillin is an antibiotic produced from penicillinum. Penicillin binding protein (PBP) is an essential enzyme used in synthesizing bacteria cell walls. When penicillin combines with PBP, synthesis of bacteria cell walls is inhibited. This is because PBP cannot supply enough proteins to synthesize bacteria cell walls after penicillin a...

AI Technical Summary

Benefits of technology

[0010]The invention provides a penicillin G biosensor comprising an extended gate field effect transistor and a tin oxide sensitive film having a penicillin G acylase film immobilized thereon to detect penicillin G concentration in a solution. The invention provides low cost, high sensitivity of ion sensitive films, accurate measurement, and rapid response time.

[0018]Additionally, physical gel entrapment immobilizes a penicillin G acylase layer, which combines the semiconductor photolithography processes. Although enzyme may loss during long detection duration, disposable biosensors may effectively solve the problem at a low cost, and suitable for large-scale production.

Problems solved by technology

This is because PBP cannot supply enough proteins to synthesize bacteria cell walls after penicillin and PBP are combined, finally resulting in breakdown and death of cells.

The chemical methods, however, have several drawbacks, for example, are expensive and complicated, and enzyme activity may easily be lost since an enzyme activity center usually participates in bonding.

Physical methods are simple and conventionally used, but also have problems of enzyme loss due to no formation of covalent bonds.

The method, however, may consume energy due to use of the pressure transducer, resulting in non-accurate measurement.

Images