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Soluble Canola Protein Isolate Production from PMM ("C307")

a technology of pmm and canola protein, which is applied in the field of production of soluble canola protein isolate, can solve the problems of poor clarity of pmm-derived canola protein isolate and general unsuitability for protein fortification of such beverages, and achieve the effect of facilitating the performance of the subsequent dilution step and reducing the viscosity of concentrated protein solution

Inactive Publication Date: 2010-02-25
BURCON NUTRASCI MB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0050]Depending on the temperature employed in the concentration step and optional diafiltration step and whether or not a pasteurization step is effected, the concentrated protein solution may be warmed to a temperature of at least about 20°, and up to about 60° C., preferably about 25° to about 40° C., to decrease the viscosity of the concentrated protein solution to facilitate performance of the subsequent dilution step and micelle formation. The concentrated protein solution should not be heated beyond a temperature above which micelle formation does not occur on dilution by chilled water.
[0051]The concentrated protein solution resu...

Problems solved by technology

The PMM-derived canola protein isolate is largely insoluble in water at natural pH and is more soluble in acid aqueous media, such as uncarbonated and carbonated beverages, including soft drinks and sport drinks, but produces solutions of poor clarity.
As such, the PMM-derived canola protein isolate is considered generally unsuitable for protein fortification of such beverages.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0072]This Example describes the preparation of a canola protein micellar mass from canola oil seed meal.

[0073]‘a’ kg of canola meal was added to ‘b’ L of ‘c’ M NaCl solution at ambient temperature and agitated for 30 minutes to provide an aqueous protein solution. The residual canola meal was removed and the resulting protein solution was partially clarified by centrifugation to produce ‘d’ L of partially clarified protein solution having a protein content of ‘e’ % by weight. The partially clarified protein solution was then filtered to further clarify resulting in a solution of volume ‘f’ having a protein content of ‘g’ by weight.

[0074]A ‘h’ L aliquot of the protein extract solution was reduced to ‘i’ kg by concentration on a polyethersulfone (PES) membrane having a molecular weight cutoff of ‘j’ daltons. The resulting concentrated protein solution had a protein content of ‘k’ % by weight.

[0075]The concentrated solution at ‘l’° C. was diluted ‘m’ fold into cold RO water having a t...

example 2

[0076]This Example describes the processing of the product from Example 1 into a canola protein isolate soluble in aqueous acid media.

[0077]‘a’ kg of the PMM from batch ‘b’ having a protein content of approximately ‘c’ wt % was resolubilized in ‘d’ L of ‘e’ M saline to provide a solution with a protein content of ‘f’ %.

[0078]The conductivity of this solution was then raised to ‘g’ mS by the addition of CaCl2 from a concentrated solution prepared by dissolving 77 wt % flake CaCl2 in RO water.

[0079]This ‘h’ L of solution was then diluted ‘i’ in ‘j’ L of room temperature RO water. Upon diluting, no pellet formed but the solution was quite cloudy. The greenish coloured precipitate was removed by centrifugation and filtration to produce ‘k’ L of clear solution having a protein content of ‘l’ wt %.

[0080]The clarified protein solution was then adjusted to pH ‘m’ with HCl. A PES membrane with a molecular weight cutoff of 10,000 Daltons was used to concentrate the pH 3 adjusted filtrate to a...

example 3

[0083]This Example describes an alternate method of processing the product from Example 1 into a canola protein isolate soluble in aqueous acid media.

[0084]‘a’ kg of PMM from batch ‘b’ was resolubilized with ‘c’ wt % (on a per protein basis) CaCl2 and ‘d’ L RO water to make a ‘e’ kg protein solution having a ‘f’ wt % protein content. The resolubilized PMM was diluted with ‘g’ volume(s) of room temperature RO water. A greenish coloured precipitate was removed by centrifugation. The resulting ‘h’ kg of centrate having a protein content of ‘i’ wt % was then adjusted to a pH of ‘j’ with HCL. The acidified clarified protein solution was then passed through ‘k’ L of granular activated carbon having the same pH and conductivity as the protein solution at a rate of ‘l’ BV / hr.

[0085]The GAC treated solution having a protein content of ‘m’ and mass of ‘n’ kg was then spray dried to produce a dry product with a protein content of ‘o’% (N×6.25) d.b. The product was designated ‘b’ C307C. The para...

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PUM

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Abstract

A soluble canola protein isolate is prepared from canola protein micellar mass by solubilizing the protein micellar mass in a calcium salt solution, preferably a calcium chloride solution, followed by dilution of the resulting canola protein solution. Following removal of the precipitate phytic acid, the aqueous canola protein solution is concentrated, optionally diafiltered, and acidified to a pH of about 2.5 to 4.0 to produce an acidified clear canola protein solution, which may be concentrated, subjected to a colour removal step and dried. The canola protein isolate so formed is soluble, transparent and heat stable in an acid aqueous environment and also is soluble at natural pH, without precipitation of protein.

Description

REFERENCE TO RELATED APPLICATION[0001]This application claims priority under 35 USC 119(e) from U.S. Provisional Patent Application No. 61 / 136,208 filed Aug. 19, 2008.FIELD OF INVENTION[0002]This invention relates to the production of soluble canola protein isolate.BACKGROUND TO THE INVENTION[0003]Canola oil seed protein isolates having protein contents of at least 100 wt % (N×6.25) can be formed from oil seed meal by a process as described in copending U.S. patent application Ser. No. 10 / 137,391 filed May 3, 2002 (U.S. Patent Application Publication No. 2003-0125526 A1 and WO 02 / 089597) and U.S. patent application Ser. No. 10 / 476,230 filed Jun. 9, 2004 (U.S. Patent Application Publication No. 2004-0254353 A1), assigned to the assignee hereof and the disclosures of which are incorporated herein by reference. The procedure involves a multiple step process comprising extracting canola oil seed meal using an aqueous salt solution, separating the resulting aqueous protein solution from ...

Claims

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Application Information

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IPC IPC(8): C07K14/415C07K1/02C07K1/14
CPCA23J1/14C07K14/415A23L2/66A23J3/14
Inventor SEGALL, KEVIN I.GREEN, BRENT E.SCHWEIZER, MARTIN
Owner BURCON NUTRASCI MB
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