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Repair of ruptured membrane by injection of naturally occurring protein in amniotic fluid sac

a technology of amniotic fluid and natural protein, which is applied in the field of perinatal medicine and obstetrics, can solve the problems of decreased risk of infection and umbilical cord compression, increased and prolonged gestation, so as to reduce the risk of infection and increase the likelihood of a mature fetus , the effect of prolonging the gestation period

Inactive Publication Date: 2010-09-23
BERRY DAVID
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0007]The present invention overcomes these and other drawbacks of the prior art by providing methods and compositions for treating premature rupture of membranes (PROM) during pregnancy. The methods and compositions provided result in prolonged gestation, decreased risk of infection and umbilical cord compression, and an increased likelihood of a mature fetus.

Problems solved by technology

The methods and compositions provided result in prolonged gestation, decreased risk of infection and umbilical cord compression, and an increased likelihood of a mature fetus.

Method used

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  • Repair of ruptured membrane by injection of naturally occurring protein in amniotic fluid sac
  • Repair of ruptured membrane by injection of naturally occurring protein in amniotic fluid sac
  • Repair of ruptured membrane by injection of naturally occurring protein in amniotic fluid sac

Examples

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Effect test

example 1

[0061]Nine inch diameter helium grade water filled balloons were initially used as an in vitro model of the amniotic membrane. The internal pressure as measured by manometry was between 25-35 cm of water. Hypodermic needles of standard medical grade were used to create puncture holes in the water filled balloons. Initially, single 20 gauge (1.0 mm) holes were created through which 5 mL of thick egg white were injected into the water filled balloons (See FIG. 1A). The hydrophobic egg white remained immiscible with water. When the egg white bolus contacted the inner surface at the leak site, the hole was successfully plugged (FIG. 1B). These seals were maintained for three weeks, at which time, the integrity of the rubber of the balloon itself began to degrade. Of note, the seal appeared more readily when the water inside the balloon was approximately equal to body temperature (37° C.) as regulated by a digital thermometer.

example 2

[0062]Repeated acetic acid elutions were performed on thick egg white. Repeated elutions with 5% acetic acid followed by washing with water led to thickened and precipitated membrane in the thick egg white. The thin egg white was easily separated from the thick egg white in the soluble form. Resuspension occurred when the pH was made basic using ammonia. Precipitation again occurred when acidic conditions were reinstated. Thus, it appears that the components of thick egg white are stable to changes in pH of the suspending solution, but the viscosity is markedly increased in acidic environments.

example 3

[0063]A dilute (5%) acetic acid was used to thicken and precipitate the thick egg white. The more viscous component was separated from the remaining egg white components. A nine-inch helium-grade balloon was then filled with water and 4 mL of acid eluted thick egg white. The balloon (inner membrane) was then placed inside a slightly water filled latex condom. This apparatus simulates the inner membrane (amnion) and outer compartment (uterus). A single 18 gauge (1.2 mm) hole was created by passing a hypodermic needle through the balloon. The thick egg white within the balloon migrated to the leak site and readily sealed the leak at the balloon / condom interface (FIG. 2). The seal was readily visualized through the transparent condom.

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Abstract

The present invention provides compositions and methods for use in treatment of premature rupture of membrane. The compositions of the invention include a therapeutic amount of an avian thick egg white composition. Preferably, the compositions include the purified thick egg white protein, ovomucin. The methods of the invention involve injecting an effective amount of the compositions into the prematurely ruptured amniotic sac of a patient in order to seal the rupture.

Description

BACKGROUND OF THE INVENTION[0001]1. Field of the Invention[0002]The present invention relates generally to the fields of obstetrics and / or perinatal medicine. More particularly the invention relates to repair of pre-parturition rupture of the amniotic fluid sac.[0003]2. Description of Related Art[0004]Premature rupture of membranes (PROM) during the second and early third trimester of human pregnancy creates a management dilemma for obstetricians. There are currently many management protocols for PROM. Recent literature argues the risks and benefits of tocolytic agents, antibiotics, and corticosteroid injections primarily for delaying delivery, preventing intraamniotic infection, and enhancing fetal maturity, respectively, in the event of almost certain preterm delivery. However, to date, no true accepted treatment for PROM exists. Medline searches of this topic reveal only scant data in Italian studies on intracervical instillation of fibrin glue, and Japanese attempts at mechanica...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K38/14A61B17/00A61B17/42A61K38/38
CPCA61B17/00491A61B17/0057A61B17/42A61K38/38A61K2300/00
Inventor BERRY, DAVID
Owner BERRY DAVID
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