Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Tissue Engineering of Lung

a tissue engineering and lung technology, applied in the field of lung tissue engineering, can solve the problems of lung being difficult to engineer in the laboratory, affecting the quality of lung tissue, and wide range of problems,

Inactive Publication Date: 2012-03-15
YALE UNIV
View PDF2 Cites 44 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0015]In one embodiment, the composition is capable of supporting and maintaining the differentiation state of an alveolar epithelial cell.

Problems solved by technology

Immunosuppression is necessary to prevent rejection of the transplanted organ, but can lead to a wide range of problems, including infection, malignancy, kidney impairment, cardiovascular problems, and neurologic disorders [Pietra et al., 2000, J Clin Invest 106:1003-10; Christie et al., 2009, J Heart Lung Transplant 28:1031-49].
However, lung is a difficult tissue to engineer in the laboratory.
Furthermore, pulmonary endothelial and epithelial cells are more difficult to culture in the laboratory than many other cell types [Maida et al., 2004, Biomaterials 25:5773-80; Reichenspumer, 2005, J Heart Lung Transplant 24:119-30], and there has been relatively slow progress in the field of pulmonary progenitor and stem cell biology [Blaisdell et al., 2009, Stem Cells 27:2263-70; Muratore et al., 2008, J Surg Res 155(2):225-30].

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tissue Engineering of Lung
  • Tissue Engineering of Lung
  • Tissue Engineering of Lung

Examples

Experimental program
Comparison scheme
Effect test

experimental examples

[0221]The invention is further described in detail by reference to the following experimental examples. These examples are provided for purposes of illustration only, and are not intended to be limiting unless otherwise specified. Thus, the invention should in no way be construed as being limited to the following examples, but rather, should be construed to encompass any and all variations which become evident as a result of the teaching provided herein.

example 1

Decellularization of Rat Lung and Morphological Characterization of Decellularized Scaffolds

[0222]A decellularized organ offers several advantages for use as a tissue engineering scaffold. In one aspect, the decellularized scaffold contains the appropriate 3-dimensional organization required for tissue function, including a vascular system and airway network in the case of lung. In addition, extracellular matrix (ECM) components are widely conserved across species, thus reducing the likelihood of a decellularized scaffold inducing an immune response upon xenogeneic implantation [Bernard et al., 1983, Biochemistry 1983; 22:5213-23]. In another aspect, native ECM offers the optimal substrate for cell attachment, spreading, growth and differentiation.

[0223]The goal of the decellularization process of the present invention is to remove cellular and nuclear material while retaining key aspects of and minimizing any damage to the ECM of the lung. The results presented herein demonstrate t...

example 2

Contribution of Extracellular Matrix Components to the Mechanical Integrity of Decellularized Lung Tissue

[0269]The following experiments were designed to evaluate the composition of the decellularized scaffolds in more detail with a focus on the mechanical properties of the scaffolds. Without wishing to be bound by any particular theory, it is believed that that decellularized lung scaffolds retain salient mechanical features of native lung, due principally to contributions from collagen and elastin. The results presented herein demonstrate the utility of the decellularized lung tissue as a platform to study lung mechanics independent of cellular contributions.

[0270]The results presented herein demonstrate that collagen content is retained, elastin content is retained at ˜40% of native levels, while glycosaminoglycans are largely lost from the decellularized scaffolds.

[0271]The materials and methods employed in these experiments are now described.

Materials and Methods

[0272]Organ Har...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Fractionaaaaaaaaaa
Fractionaaaaaaaaaa
Fractionaaaaaaaaaa
Login to View More

Abstract

The present invention relates to compositions comprising a decellularized tissue. The present invention also provides an engineered three dimensional lung tissue exhibiting characteristics of a natural lung tissue. The engineered tissue is useful for the study of lung developmental biology and pathology as well as drug discovery.

Description

BACKGROUND OF THE INVENTION[0001]Every year, 400,000 Americans die of lung disease. Of further concern, the death rate due to lung disease is increasing, while the death rates for the other major disease categories are decreasing (heart disease, cancer and stroke). For several lung diseases, including cystic fibrosis, emphysema / COPD, and idiopathic pulmonary fibrosis, lung transplantation remains the only definitive treatment. However, patient survival after lung transplant is only 50% at 5 years and 24% at 10 years [Mondrinos et al., 2008, Tissue Eng 14:361-8]. There is therefore great demand for the development of engineered lung tissue that could be used for transplantation. One advantage of engineered lung tissue is that the tissue can be grown using a patient's own cells, thereby avoiding the need for strong immunosuppression, as is required with current lung transplantation. Immunosuppression is necessary to prevent rejection of the transplanted organ, but can lead to a wide r...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K35/42A61P11/00C12Q1/68C12N5/071C12Q1/02
CPCA61K35/42C12N2533/90C12N5/0688A61P11/00A61P43/00
Inventor CALLE, ELIZABETHNIKLASON, LAURA E.PETERSON, THOMASGUI, LIQIONG
Owner YALE UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com