Linker, impedance biochip, and method of quantitatively detecting target analyte in fluid sample using the biochip
a biochip and target analyte technology, applied in the field of linkers on biochips, can solve the problems of decreasing or absence of signal, and the conventionally adopted linker is not suitable anymore, and achieves the effects of increasing faradaic current, reducing cost, and being more compa
Active Publication Date: 2012-03-22
NAT TAIWAN UNIV
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Benefits of technology
[0013]According to the embodiment of the present invention, the biochip which adopts the linker comprising a compound of formula (I), provides a more electrically accessible surface to the redox species, so as to increase the faradaic current induced by the redox species. The advantage of increasing faradaic current is that the electronic circuit for measuring the impedance of the biochip surface can be designed more compact and at lower cost, and thus is more suitable for point-of-care applications.
Problems solved by technology
The capacitance biochip has a drawback that forming a defect-free bio-recognition layer is more crucial than that in the faradaic biochip, because if the defect-free boo-recognition layer is not well insulated, ions can move through a defect area, and cause short circuiting leading to a decrease or absence of the signal.
Method used
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Electrochemical Characterization
[0060]The molecules used as the linker or in the linker are shown in Table 1.
TABLE 1Short MolecularnameweightStructureS2TA240A2T3S268CS23S284CPD336
[0061]The electrolyte for the electrochemical characterization was a 1× PBS, pH 7.2 with 1 mM K3Fe(CN)6 / K4Fe(CN)6 (Sigma-Aldrich) mixing solution and was prepared immediately before usage. The molecules as the linker or part of the linker,[0062]5′-(mercapto)-[2,2′-bithiophene]-5-carbaldehyde (and[0063]5′-(mercapto)-[2,2′-bithiophene]-5-carboxylic acid),[0064]5′-(mercaptomethyl)-[2,2′-bithiophene]-5-carbaldehyde (S2TA) (and[0065]5′-(mercaptomethyl)-[2,2′-bithiophene]-5-carboxylic acid),[0066]5′-(3-mercaptopropyl)-[2,2′-bithiophene]-5-carbaldehyde (A2T3S) (and[0067]5′-(2-mercaptoethyl)-[2,2′-bithiophene]-5-carbaldehyde),[0068]5′-(3-mercaptopropyl)-[2,2′-bithiophene]-5-carboxylic acid (CS23S) (and[0069]5′-(2-mercaptoethyl)-[2,2′-bithiophene]-5-carboxylic acid), and[0070]5′-(6-mercaptohexyl)-[2,2′-bithiophene]-...
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Abstract
The present invention provides a linker for joining an electrode and a capture probe on a biochip, and a biochip comprising the linker. The impedance baseline of the linker of the present invention is three orders lower than the conventional long chain thiol linker when adopting in a fadaraic impedance biochip construction. With lower impedance baseline, the device designed to measure the signal of the biochip of the present invention could be further simplied on the electrical circuit design and be made in lower cost, compacter size and get the potential to be used in point-of-care applications. The present invention also provides a method of quantitatively detecting a concentration of a target analyte in a fluid sample by adopting the biochip and the linker of present invention.
Description
BACKGROUND OF THE INVENTION[0001]1. Field of the Invention[0002]The present invention relates to linkers on biochips, and more particularly, to a linker of bridging an electrode and a capture probe on a biochip.[0003]2. Description of Related Art[0004]A biochip is a chip designed to detect or quantify a target analyte such as protein, DNA, cell, glucose, cardiomyopathic biomarkers (e.g., S100 and C-Reactive protein (CRP), Troponin I, CKMB and the like), cancer biomarkers (e.g., cancer antigen 125 (CA125), carcinoembryonic antigen (CEA), prostate-specific antigen (PSA) and the like), bacterium biomarkers (e.g., E. Coli, staphylococcus and the like), and virus (e.g., H1N1 and the like. Many biochips are affinity-based that use an immobilized capture probe to bind the target analyte and then use a transducer to detect a change at a localized surface. The capture probe is a bio-recognition element that could bind or interact with the target analyte. For example, the capture probe may be...
Claims
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Patent Timeline
Login to View More IPC IPC(8): G01N27/327G01N33/50C07D409/04
CPCC07D333/18C07D333/22C07D333/34G01N33/5438C07D409/04C07D409/14G01N33/54353C07D333/38
Inventor LEE, CHIH-KUNGLEE, ADAM SHIH-YUANCHEN, CHING-SUNGCHANG, KU-NINGCHEN, YING-HUALEE, BRYAN YONG-JAY
Owner NAT TAIWAN UNIV