Collagen extraction from aquatic animals
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[0020]Red tilapia (Oreochromis nilotica) is a cultured freshwater fish obtained from a local fish farm in Ulu Langat, Selangor. The red tilapia weighed between 500 g to 600 g. Upon arrival at the laboratory, the fish were killed, filleted and the skin was manually removed. The skins were stored at −20° C. until to be used.
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[0021]Pepsin (crystallized and lyophilized, EC 3.4.23.1, from porcine stomach mucosa) with a declared activity of 3460 units / mg protein was obtained from Sigma Chemical. Trypsin (crystallized and lyophilized, EC 3.4.21.4 from porcine pancreas) with a declared activity of 40 U / mg protein and Papain (dry powder, EC 3.4.22.2, from Carica papaya) with a declared activity of 30000 USP-U / mg were obtained from Merck (USA). All other chemicals used were of analytical grade.
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