Methods and Compounds for Promoting Vessel Regression
a technology of regression and hvs, applied in the field of methods and compounds for promoting vessel regression, can solve the problems of permanent blindness if left untreated, degeneration of hvs, etc., and achieve the effect of improving the parameter
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Benefits of technology
Problems solved by technology
Method used
Image
Examples
example 1
Observation of Whole-Mount Specimens
[0095]To document the changes in the hyaloid vascular system associated with development in the cornea, whole-mount specimens were observed.
[0096]A total of 44 C57BL / 6 newborn male and female litters were obtained from Jackson Laboratories (Bar Harbor, Me.) were used for the experiments described herein. The age of the newborn mice was expressed in terms of post-gestational days. The newborn mice were sacrificed on post-gestational days 1, 4, 8, 16, 24, and 30 (i.e., P1, p4, p16, p24, and P30). Four eyes per each post-gestational day were used for the histological study. For the lens and vitreous protein extraction were used 20 eyes of P 1 mice, 20 eyes of P 4 mice, 12 eyes of P 8 mice and 12 eyes of P 16 mice. Each of the 12 newborn mice used for the histological study was perfused with 4% paraformaldehyde in PBS under general anesthesia administered by intramuscular injection of a mixture of ketamine (200 mg / kg) and xylazine (10 mg / kg). The eyes...
example 2
Differential Expression of Activin Receptor-Like Kinase 1 (ALK1)
[0138]The activin receptor-like kinase 1 (ALK1), a TGF-beta1 type I receptor, plays an inhibitory role in angiogenesis and vascular development. Mutations of ALK1 gene are linked to human type II hereditary hemorrhagic telangiectasia. Our purpose was to develop a mouse model to study the differential expression of proteins during the phase of Hyaloid Vascular System (HVS) regression and determine the role of ALK1 in this model.
[0139]Materials and Methods: Thirty-two newborn C57BL / 6 mice were sacrificed on post-gestational days 1 (n=20 eyes), 4 (n=20 eyes), 8 (n=12 eyes), and 16 (n=12 eyes). The lens, the Pupillary Membrane (PM), the Tunica Vasculosa Lentis (TVL) and the primary vitreous containing the Vasa Hyaloidea Propria (VHP) were isolated. Proteins were extracted from each specimen, loaded onto nonlinear immobilized pH gradient (IPG) gel strips, and separated by isoelectric points and molecular weights. Protein exp...
example 3
Progressive Regression of HVS in the Mouse and the Differential Protein Expression Profile
[0143]A time course experiment was designed to examine the changes in protein expression at P1 and P16 throughout the HVS regression process. As this is the first reported application of proteomic analysis on HVS regression, several conditions for tissue selection and preparation were developed and optimized to obtain clean specimens of the lens and vitreous for 2-DE gels.
[0144]Mouse Anterior Tissue Preparation for 2-DE Gels
[0145]For the protein extraction and ALK1 immunolocalization studies, newborn mice were sacrificed on post-natal days 1, 4 and 16. Using a dry ice bed, frozen mouse eyes were scraped with a blade (Beaver #15) to remove the cornea, conjunctiva, sclera, ciliary body, uvea, and retina, leaving the lens surrounded by the PM, TVL, and primary vitreous (containing VHP). The dry ice bed was used to prevent warming of the specimen, tissue melting, and subsequent protein denaturation...
PUM
| Property | Measurement | Unit |
|---|---|---|
| Fraction | aaaaa | aaaaa |
| Atomic weight | aaaaa | aaaaa |
| Disorder | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More