Unlock instant, AI-driven research and patent intelligence for your innovation.

Dried hydrogel, dried vitrigel membrane, and methods for producing the same

a technology of vitrigel and hydrogel, which is applied in the field of drying hydrogel and drying vitrigel membrane, and methods for producing the same, and can solve the problems of difficult handling, difficult phase-contrast microscopic observation of cultured cells, and not being widely spread

Inactive Publication Date: 2013-08-22
NAT INST OF AGROBIOLOGICAL SCI +2
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention allows for the quick and efficient production of dried hydrogel and vitrigel membrane with precise shapes. Additionally, by using a releasable film, the dried membrane or hydrogel can be easily handled and worked on in a membrane state. The invention also allows for the performance of cutting work on the dried membrane or hydrogel in a variety of forms, other than the shape of a wall surface mold.

Problems solved by technology

In particular, a three-dimensional culture technology using a collagen gel for a cell culture carrier is useful for reconstructing a neovascularization model, a cancer infiltration model, an epithelial-mesenchymal model, and the like; however, it has not been widely spread yet.
As for the reasons therefor, it has been considered that since the conventional collagen gel is constituted of low-density fibers, it is so soft that its handling is difficult; since the conventional collagen gel is opaque, the phase-contrast microscopic observation of cultured cells is not always easy; and the like.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Dried hydrogel, dried vitrigel membrane, and methods for producing the same
  • Dried hydrogel, dried vitrigel membrane, and methods for producing the same
  • Dried hydrogel, dried vitrigel membrane, and methods for producing the same

Examples

Experimental program
Comparison scheme
Effect test

example 1

Fabrication of Ring-Shaped Nylon Membrane Support-Provided Dried Collagen Vitrigel Membrane (Collagen Amount 0.55 to 2.2 mg / cm2) Utilizing Substrate and Wall Surface Mold

[0136]A 0.25% sol of bovine skin-derived type I collagen was prepared by uniformly mixing equal amounts of a 0.5% type I collagen acidic solution (KOKEN #IAC-50; 5 mg / mL) and a cell culture medium within a sterlized conical tube having a capacity of 50 mL (Falcon #35-2070) cooled on ice. Incidentally, the cell culture medium as used herein is a Dulbecco's modified Eagle's culture medium (GIBCO BRL #11885-084) containing 10% inactivated fetal calf serum (SIGMA #F2442), 20 mM of HEPES (GIBCO BRL #15630-080), 100 units / mL of penicillin, and 100 μg / mL of streptomycin (GIBCO BRL #15140-148).

[0137]The ring-shaped nylon membrane support was fabricated by cutting out a nylon membrane (GE Healthcare #RPN1732B; Hybond-N+, nucleic acid blotting membrane) so as to have a diameter of an outer circle of 33 mm and a diameter of an...

example 2

Fabrication of Dried Collagen Vitrigel Membrane (Collagen Amount: 0.52 to 2.1 mg / cm2) Adsorbed onto Parafilm Utilizing Parafilm Laid on Substrate and Wall Surface Mold

[0157]A bottom surface of a hydrophobic polystyrene-made culture Petri dish with a diameter of 60 mm (Falcon #35-1007) was used as a substrate. In addition, an acrylic resin-made mold with a diameter of an outer circle of 39 mm, a diameter of an inner circle of 35 mm, and a height of 10.0 mm was used as a wall surface mold. Parafilm (manufactured by Pechiney Plastic Packaging) was cut in a circle with a diameter of 50 mm and used. Incidentally, each of the wall surface mold and the Parafilm was used after subjecting to a sterilization treatment by spraying 70% ethanol and then wiping it off. Specifically, one sheet of Parafilm cut in a circle with a diameter of 50 mm was laid on the bottom surface of the hydrophobic polystyrene-made culture Petri dish with a diameter of 60 mm, and one wall surface mold was placed there...

example 3

Quick Mass-Production Method of Ring-Shaped Nylon Membrane Support-Provided Dried Collagen Vitrigel Membrane Utilizing Substrate and Wall Surface Mold

[0166]A bottom surface of a hydrophobic polystyrene-made culture Petri dish of 100 mm×100 mm (Falcon #35-1112) was used as substrate. In addition, four acrylic resin-made molds with a diameter of an outer circle of 38 mm, a diameter of an inner circle of 34 mm, and a height of 10.0 mm were used as a wall surface mold. In addition, the wall surface molds were used after subjecting to a sterilization treatment by spraying 70% ethanol and then wiping it off.

[0167]Specifically, four wall surface molds were placed on one substrate, thereby fabricating four containers capable of separating the substrate and the wall surface mold from each other. One sheet of a ring-shaped nylon membrane support was inserted into each of the containers, 2.0 mL of a 0.25% collagen sol was injected, and the substrate was covered with a lid of the Petri dish, fo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
thicknessaaaaaaaaaa
thicknessaaaaaaaaaa
Login to View More

Abstract

A dried vitrigel membrane is produced by a method including the following steps of (1) a step of keeping a hydrogel in the inside of a wall surface mold with a shape the same as the desired shape disposed on a substrate, and discharging a part of free water within the hydrogel from a gap between the substrate and the wall surface mold; (2) a step of removing the wall surface mold from the top of the substrate; (3) a step of drying the hydrogel to remove the residual free water, thereby fabricating a vitrified dried hydrogel; (4) a step of rehydrating the dried hydrogel to fabricate a vitrigel membrane; and (5) a step of redrying the vitrigel membrane to remove free water, thereby fabricating a vitrified dried vitrigel membrane.

Description

TECHNICAL FIELD[0001]The present invention relates to a dried hydrogel not having an amorphous outer peripheral edge, a dried vitrigel membrane, and methods for producing the same.BACKGROUND ART[0002]For studies of drug design, the development of culture systems capable of simply constructing three-dimensional culture models reflecting living bodies using various functional cells has been long demanded. In particular, a three-dimensional culture technology using a collagen gel for a cell culture carrier is useful for reconstructing a neovascularization model, a cancer infiltration model, an epithelial-mesenchymal model, and the like; however, it has not been widely spread yet.[0003]As for the reasons therefor, it has been considered that since the conventional collagen gel is constituted of low-density fibers, it is so soft that its handling is difficult; since the conventional collagen gel is opaque, the phase-contrast microscopic observation of cultured cells is not always easy; a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): C12N5/00B29D7/01
CPCC12M25/14B29D7/01B29K2089/00B29C39/02B29C41/02C12N5/0062
Inventor TAKEZAWA, TOSHIAKIOSHIKATA, AYUMIKUROYAMA, HIROYUKISAWAGUCHI, TOMOYAYAMAGUCHI, HIROYUKI
Owner NAT INST OF AGROBIOLOGICAL SCI