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Porous solid phase for rapidly isolating biological molecules for nucleic acid amplification reaction from biological sample, and use thereof

Inactive Publication Date: 2015-09-10
BIO CUBE SYST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The patent allows for a quick and accurate way to detect a specific part of a biological sample. This can be useful for things like detecting amplified DNA, synthesizing cDNAs, or identifying inflammation caused by a pathogen.

Problems solved by technology

In the case when PCR is used for the diagnosis of pathogens, it may have a difficulty in examining a large amount of a sample at one trial although PCR has high specificity and detection sensitivity.
This is because the rapid separation of a PCR template from various specimens requires a considerable amount of time and costs.
However, these methods have drawbacks that the extraction efficiency of the genetic material is not uniform, and reproducibility of PCR reaction is low.

Method used

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  • Porous solid phase for rapidly isolating biological molecules for nucleic acid amplification reaction from biological sample, and use thereof
  • Porous solid phase for rapidly isolating biological molecules for nucleic acid amplification reaction from biological sample, and use thereof
  • Porous solid phase for rapidly isolating biological molecules for nucleic acid amplification reaction from biological sample, and use thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

Adsorption of a Genetic Material Using a Porous Ceramic Cube and its Use as a Template for RT-PCR / PCR

[0068]The adsorption of a genetic material using a porous ceramic cube of the present invention and a diagram illustrating its use as a template for RT-PCR / PCR is shown in FIG. 1. In the present invention, 14 kinds of cubes were manufactured by varying the manufacturing temperature using the main components described in Table 1 so that the size of pores on the surface of the 1 mm3 cube can vary, and the enlarged view of the porous ceramic cube is shown in FIG. 1B. Since cubes are porous smaller substances than the pores can be sucked. The present invention aims at selectively absorbing the desirable ones among various substances generated during rupture of tissues by varying the size of pores (In general, when ceramics are manufactured at high temperature the pore size becomes smaller), that is, providing a cube with an ultrafiltration function to be capable of maximally excluding PC...

example 2

Isolation of a DNA / RNA Template for Nucleic Acid Amplification from a Biological Sample Using a Cut-Out Ceramic Fragment

[0070]Seven kinds of cut-out ceramic fragments including blue and white porcelain was broken into small pieces using a nipper, and the fragments with a volume of about 1 mm3 selected from the unglazed area were used as an absorptive material for a genetic material. For the 5th ceramic fragment in FIG. 2A, it was divided into yellow and grey according to the area coated with glaze, and the yellow fragments and the grey fragments obtained respectively therefrom were indicated as 5 and 6, respectively. Each ceramic fragment was placed on top of Capsicum annuum CM334 pepper leaves, lightly pressed with a flat portion on the rear side of metal tweezers to obtain gDNA. One ceramic fragment keeping gDNA was added into each PCR tube, which was already added with a primer premix capable of verifying molecular markers related to the trichomes of pepper. The PCR premix consis...

example 3

Examination of an Absorption Rate of Biological Molecules According to the Types of Porous Ceramic Cubes of Oxide Material

[0073]The picture of the surfaces of the respective porous ceramic cube manufactured using the oxide described in Table as a main component enlarged under scanning electron microscope (SEM) is shown in FIG. 3. Since the surface and pore size vary according to the main components of ceramics and their manufacturing temperature, they should be manufactured to obtain the optimum pore size and number of the ceramic cubes in order to improve the absorption rate of the target biological molecules.

TABLE 1SignMain ComponentManufacturing Temperature (° C.)1Al2O314502Al2O315503Fe2O38004Fe2O38505Fe2O39006LTCC6507LTCC7508LTCC8509PbO100010PbO115011PbO125012ZnO80013ZnO90014ZnO1000

[0074]① Regarding Total RNA Isolated from CMV-Infected Capsicum annuum CM334 Pepper Leaves and Genomic DNA Isolated from CM334 Pepper

[0075]In order to examine the absorption efficiency of biological m...

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Abstract

The present invention provides a method for rapidly ascertaining whether or not a target sequence exists in a biological sample by: rapidly isolating biological samples for a nucleic acid amplification reaction from the biological sample by using a porous solid phase capable of rapidly sucking biological samples present in a biological sample into pores; and amplifying the same.

Description

TECHNICAL FIELD[0001]The present invention relates to a porous solid phase for rapidly isolating biological molecules for a nucleic acid amplification reaction from a biological sample, and a use thereof. More specifically, the present invention relates to a method for rapidly isolating biological molecules for a nucleic acid amplification reaction from a biological sample including putting a porous solid phase in contact with a biological sample to get biological molecules present in the biological sample sucked into the pores of the porous solid phase; a method for amplifying a target sequence in a biological sample including directly adding a porous solid phase, into the pores of which biological molecules present in a biological sample are sucked by putting the porous solid phase in contact with the biological sample, or performing a reverse transcriptase reaction followed by amplifying a target sequence using the porous solid phase as a template for a nucleic acid amplification...

Claims

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Application Information

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IPC IPC(8): C12N15/10C01F7/02C01G21/06C01G9/02C12Q1/68C01G49/06
CPCC12N15/1006C12Q1/6806C12Q1/686C01F7/02C01G21/06C01G9/02C01G49/06C12Q2523/308G01N33/48
Inventor PARK, DAEHOSHIN, JUNSUNGHAN, JUNG-HEONIN, GUEM SUKCHOI, HONG SOOKIM, JEONG SOO
Owner BIO CUBE SYST
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