Extracts of curcuma amada and uses thereof

Abstract

The invention concerns carbon dioxide extracts of Curcuma amada (mango ginger), including supercritical carbon dioxide extracts of C. amada; methods for their production; compositions comprising the extracts; methods for treating or delaying the onset of conditions such as cell proliferation disorder (e.g., cancer), inflammation, infection, hyperlipidemia, hypercholesterolemia, hypertriglyceridemia, hyperglycemia, platelet hyper-aggregation, immune disorder such as autoimmune disorder, or neurodegenerative condition; and methods for inhibiting expression of Bcl-2, Bak, and p53 genes; inhibiting expression of the COX-2 and NF-kB genes, inhibiting production of phosphorylated target of rapamycin (TOR), modulating AMP-activated protein kinase (AMPK), inhibiting protein kinase B (AKT) signaling, modulating the Ras / Raf / MEK / ERK signaling pathway, and modulating the Ras / PI3K / PTEN / Akt / mTOR signaling pathway. Another aspect of the invention concerns a method for inhibiting contamination, comprising applying the extract or composition of the invention to a surface. Another aspect of the invention concerns a method for promoting longevity of a cell in vitro or in vivo, comprising contacting a target cell in vitro or in vivo with an effective amount of the extract or composition of the invention. Another aspect of the invention concerns a method for promoting longevity of a subject, comprising administering an effective amount of the extract or composition of the invention. Another aspect of the invention concerns a method for inhibiting the metabolism of a cancer cell, comprising contacting the target cancer cell in vitro or in vivo with an effective amount of the extract or composition of the invention. Another aspect of the invention concerns a kit including the extract or composition; a container containing the extract or composition; and packaging material.

Description

CROSS-REFERENCE TO RELATED APPLICATION[0001]The present application claims the benefit of U.S. Provisional Application Ser. No. 62 / 110,062, filed Jan. 30, 2015, which is hereby incorporated by reference herein in its entirety, including any figures, tables, nucleic acid sequences, amino acid sequences, or drawings.BACKGROUND OF THE INVENTION[0002]The genus Curcuma contains over 80 species of rhizomatous herbs of which many have significant value as medicines, dyes and spices (Islam 2004). Curcuma amada Roxb. is an important member of the genus commonly known as mango ginger due to the raw mango-like aroma of the rhizome. It has a morphological and phylogenic resemblance with ginger (Zingiber officinale) but imparts mango (Mangifera indica) flavor. Mango flavor is in part attributed to cis-ocimene among the 68 volatile aroma components present in the essential oil of mango ginger rhizome (Achut and Bandyopadhyaya 1984; Srinivas et al. 1989; Singh et al. 2002; Singh et al. 2003). Ethn...

AI Technical Summary

Benefits of technology

The patent text describes a method for extracting a carbon dioxide extract from the rhizomes of Curcuma amada using supercritical carbon dioxide or liquid carbon dioxide extraction. The extract has superior anticancer activity against human rhabdomyosarcoma and glioblastoma cell lines in culture and can be used in combination with other chemotherapy agents. The extract contains a combination of compounds, including LDD, α-pinene, camphene, β-pinene, β-myrcene, limonene, β-cariophyllene, ar-curcumene, and α-zingiberene. The extract can also be produced with no more than 10% ethanol as a co-solvent. The patent also describes a composition of the extract or a combination of the extract with other agents, such as a natural or synthetic oil, a botanical extract, or an antioxidant. The composition can be used as a therapy or preventive treatment for various conditions such as cancer, arteriosclerotic vascular disease, inflammation, fever, infection, hyperlipidemia, hypercholesterolemia, hypertriglyceridemia, hyperglycemia, platelet hyper-aggregation, aged and sun-damaged skin, immune disorder, or neurodegenerative conditions. The extract or composition can be administered alone or in combination with other treatments.

Problems solved by technology

Where ethanol is used as a co-solvent, LDD is largely destroyed if ethanol is distilled from the resulting extract.

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