Method for producing polyisoprenoid, transformed plant, method for producing pneumatic tire and method for producing rubber product
a technology of polyisoprenoid and transformed plant, which is applied in the direction of transferase, peptide sources, other domestic articles, etc., can solve the problems of insufficient understanding of the association between cpt and cpt, limited period during which natural rubber can be extracted, and difficult to greatly increase the production of natural rubber using hevea brasiliensis, etc., to achieve the effect of enhancing the activity of the cpt family protein, enhancing the activity of rubber
Active Publication Date: 2017-02-23
SUMITOMO RUBBER IND LTD +1
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Benefits of technology
[0057]The method for producing a polyisoprenoid of the first invention includes a step of binding a protein expressed by a gene coding for a cis-prenyltransferase (CPT) family protein, a protein expressed by a gene coding for a Nogo-B receptor (NgBR) family protein, and a protein expressed by a gene coding for a rubber elongation factor (REF) family protein to rubber particles in vitro. Binding the CPT family protein, NgBR family protein, and REF family protein to rubber particles is expected to stabilize and enhance the activity of the CPT family protein, thereby enhancing the rubber synthesis activity of rubber particles. Thus, it is possible to produce rubber more efficiently in reaction vessels (e.g. test tubes, industrial plants).
[0058]The method for producing a pneumatic tire of the first invention includes the steps of: kneading a polyisoprenoid produced by the method for producing a polyisoprenoid of the first invention with an additive to obtain a kneaded mixture; building a green tire from the kneaded mixture; and vulcanizing the green tire. With this method, a pneumatic tire is produced from a polyisoprenoid produced by a method that produces a polyisoprenoid with high productivity. Thus, it is possible to use plant resources effectively to produce an environmentally friendly pneumatic tire.
[0059]The method for producing a rubber product of the first invention includes the steps of: kneading a polyisoprenoid produced by the method for producing a polyisoprenoid of the first invention with an additive to obtain a kneaded mixture; forming a raw rubber product from the kneaded mixture; and vulcanizing the raw rubber product. With this method, a rubber product is produced from a polyisoprenoid produced by a method that produces a polyisoprenoid with high productivity. Thus, it is possible to use plant resources effectively to produce an environmentally friendly rubber product.
[0060]The method for producing a polyisoprenoid of the second invention includes producing a polyisoprenoid in a transformed pl
Problems solved by technology
Moreover, Hevea brasiliensis requires about seven years from planting to mature enough for rubber extraction, and the period during which natural rubber can be extracted is limited to 20 to 30 years.
Although more natural rubber is expected to be demanded mai
Method used
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example 1
Extraction of Total RNA from Hevea Latex
[0245]Total RNA was extracted from the latex of Hevea brasiliensis by the hot phenol method. To 6 mL of the latex were added 6 mL of 100 mM sodium acetate buffer and 1 mL of a 10% SDS solution, and then 12 mL of water-saturated phenol pre-heated at 65° C. The mixture was incubated for 5 minutes at 65° C., agitated in a vortex, and centrifuged at 7000 rpm for 10 minutes at room temperature. After the centrifugation, the supernatant was transferred to a new tube, 12 mL of a phenol:chloroform (1:1) solution was added, and the mixture was agitated by shaking for 2 minutes. After the agitation, the resulting mixture was centrifuged again at 7000 rpm for 10 minutes at room temperature, the supernatant was transferred to a new tube, 12 mL of a chloroform:isoamyl alcohol (24:1) solution was added, and the mixture was agitated by shaking for 2 minutes. After the agitation, the resulting mixture was centrifuged again at 7000 rpm for 10 minutes at room t...
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Abstract
Provided is a method for producing a polyisoprenoid, which can increase natural rubber production by enhancing the rubber synthesis activity of rubber particles. The present invention provides methods for producing a polyisoprenoid using a gene coding for a cis-prenyltransferase (CPT) family protein, a gene coding for a Nogo-B receptor (NgBR) family protein and a gene coding for a rubber elongation factor (REF) family protein, specifically a method for producing a polyisoprenoid in vitro using rubber particles bound to proteins coded for by these genes, and a method for producing a polyisoprenoid in vivo using a recombinant organism (plant) having these genes introduced therein.
Description
TECHNICAL FIELD[0001]The present invention relates to a method for producing a polyisoprenoid, a transformed plant, a method for producing a pneumatic tire, and a method for producing a rubber product.BACKGROUND ART[0002]Nowadays natural rubber (one example of polyisoprenoids) for use in industrial rubber products are obtained by cultivating rubber-producing plants, such as para rubber tree (Hevea brasiliensis) belonging to the family Euphorbiaceae, or Indian rubber tree (Ficus elastica) belonging to the family Moraceae, to biosynthesize natural rubber by the lactiferous cells of the plants, and harvesting the natural rubber by hand from the plants.[0003]At present, Hevea brasiliensis is practically the only one source of natural rubber for industrial rubber products. Hevea brasiliensis is a plant that can grow only in limited areas such as in Southeast Asia and South America. Conventionally, natural rubber production has been increased by applying ethephon or methyl jasmonate to He...
Claims
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Patent Timeline
Login to view more IPC IPC(8): C12P5/00B29D30/06B29D30/08C12N15/82C12N9/10
CPCC12P5/007C12N15/8243C12N9/1085B29K2607/00B29D30/0601C12Y205/0102B29D30/08C07K14/415
Inventor INOUE, YUKINOYAMAGUCHI, HARUHIKOFUSHIHARA, KAZUHISATAKAHASHI, SEIJIYAMASHITA, SATOSHINAKAYAMA, TORU
Owner SUMITOMO RUBBER IND LTD
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