Method and apparatus for producing container, cell culture vessel, method for culturing cells, method for producing cell culture vessel, and apparatus for producing cell culture vessel

Inactive Publication Date: 2019-05-30
TOYO SEIKAN GRP HLDG LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0036]According to the present invention, a vessel with one or more depressions formed in an inner surface thereof can be efficiently manufactured. It is also possible to efficiently manufacture a vessel which, especially if applied for use in cell culture, can appropriately maintain the density of cells during culture to suppress depletion of culture medium ingredients needed for their proliferation and can also suppress any contamination risk with contaminants and the like.
[0037]According to the present invention, it is also possible to ensure the

Problems solved by technology

Therefore, no efficient proliferation and differentiation induction of cells can be performed if the density of the cells in culture medium is too low.
Nonetheless, general cell culture bags involve a proble

Method used

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  • Method and apparatus for producing container, cell culture vessel, method for culturing cells, method for producing cell culture vessel, and apparatus for producing cell culture vessel
  • Method and apparatus for producing container, cell culture vessel, method for culturing cells, method for producing cell culture vessel, and apparatus for producing cell culture vessel
  • Method and apparatus for producing container, cell culture vessel, method for culturing cells, method for producing cell culture vessel, and apparatus for producing cell culture vessel

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first embodiment

[Cell Culture Vessel 1]

[0062]A cell culture vessel 1 depicted in FIGS. 1A to 1C has a plurality of depressions in a bottom wall, and includes a vessel main body 2 and a charge / discharge port 3. The vessel main body 2 is formed of a known plastic film having gas permeability, and the charge / discharge port 3 is formed of a tubular member through which culture medium, cells, and the like can flow.

[0063]The vessel main body 2 is sealed at a peripheral edge portion thereof, has a bulge shape protruding in a plateau shape on the side of a top wall 2a thereof, and is formed such that the top wall 2a formed as a flat wall is inclined at an edge thereof to extend to the peripheral edge portion. In addition, the vessel main body 2 includes, in a bottom wall 2b thereof, depressions 4 to be used as cell culture portions. At least one depression is required although the plurality of depressions 4 is included in this embodiment. No particular limitation is imposed on the size of the vessel main b...

second embodiment

[0102]A second embodiment of the present invention will next be described with reference to FIGS. 6 to 8.

[0103]A manufacturing apparatus 30 for the cell culture vessel in the second embodiment is different from the manufacturing apparatus 20 in the first embodiment, for example, in that the vessel support member 23 includes a suction channel 23b, a suction device 27 is included, and temperature control devices 28 are also included.

[0104]Therefore, these differences from the first embodiment will hereinafter be described primarily, and components with the same configurations or functions as in the first embodiment will be identified by the same signs as in the first embodiment, and their description will be omitted as desired (this will equally apply to other embodiments and modifications to be described later).

[0105]The manufacturing apparatus 30 will be described as a configuration including both the suction device 27 and the temperature control devices 28 in this embodiment, but t...

third embodiment

[0127]A third embodiment of the present invention will next be described with reference to FIG. 9.

[0128]A manufacturing apparatus 40 for the cell culture vessel in the third embodiment is different from the manufacturing apparatus 20 in the first embodiment, for example, in that the placement stage main member 22 on which the vessel support member 23 is not mounted serves as a placement stage T and through-holes 22a are formed as substitutes for the concave portions 23a in the placement stage T. In this embodiment, the through-holes are not necessarily required and desired holes other than the through-holes may be formed in the placement stage T.

[0129]In other words, the manufacturing apparatus 40 for the cell culture vessel as depicted in FIG. 9 is configured including the placement stage main member 22 with the through-holes 22a formed therein. The through-holes 22a correspond to the concave portions 23a in the first embodiment, and have a shape extending through the placement sta...

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Abstract

A vessel manufacturing method includes placing a bag-shaped, film-based vessel on a placement stage with concave portions formed on the stage, introducing fluid into the vessel placed on the stage, and pressing the vessel which is placed on the stage, by a pressing member while heating at least one of the stage and the pressing member. Meanwhile, a cell culture vessel includes a first vessel wall as a bottom wall and a second vessel wall. The first vessel wall is formed of a flat film having gas permeability. The second vessel wall is disposed in contact with a peripheral edge portion of the first vessel wall, and has a bulge shape protruding relative to the first vessel wall. The first vessel wall is flat at its section other than a region where the first vessel wall is in contact with at least a charge/discharge port.

Description

TECHNICAL FIELD[0001]The present invention relates to a method and apparatus for manufacturing a vessel that can store various liquids and the like. The present invention also relates to techniques for culturing various cells, more specifically to a cell culture vessel that has gas permeability and can culture cells, a cell culturing method using the cell culture vessel, and a method for manufacturing the cell culture vessel.BACKGROUND ART[0002]In the modern medical field typified by gene therapy and regenerative therapy, it is practiced to conduct culture and differentiation induction of objective cells, which include tissues, microorganisms, viruses, and the like, under artificial environments. In recent years, there is a requirement especially for efficient and bulk culture and differentiation induction of the above-mentioned cells under artificial environments.[0003]Now, upon culture and differentiation induction of cells, it is important to maintain the density of the cells in ...

Claims

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Application Information

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IPC IPC(8): C12M1/12C12M1/00
CPCC12M25/08C12M23/58C12M47/04C12M23/02C12M23/12C12M23/14C12M23/22C12M23/24C12M41/36B29C35/007B29C59/02B65B61/24C12M1/00C12M3/00
Inventor SUENAGA, RYOTANAKA, SATOSHITOTANI, TAKAHIKOTAKAHASHI, NAOKI
Owner TOYO SEIKAN GRP HLDG LTD
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