Shape Memory Silk Materials
a memory silk and material technology, applied in the field of memory silk materials, can solve problems such as shape deformation
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example 1
[0289]The present example describes a silk material that rapid re-swelling after deformation triggered by the presence of aqueous media like water or PBS in accordance with some embodiments of the present disclosure.
Methodology:
[0290]Cocoons of Bombyx mori were boiled for either 10 or 20 minutes in an aqueous solution of 0.02 M sodium carbonate and then rinsed with pure water. The extracted silk fibroin was dried for 12 hours in a chemical hood before being dissolved in 9.3 M LiBr solution at 60° C. for 4 hours, yielding a 20% (w / v) solution. This solution was dialyzed against distilled water using Pierce Slide-a-Lyzer cassettes, MWCO 3500 Da (Rockford, Ill.) for 3 days to remove LiBr. The solution was centrifuged to remove aggregates that formed during purification. The final concentration of aqueous silk fibroin was between 6-8% (w / v). This concentration was diluted with pure water down to 3% (w / v) for all experiments.
[0291]The boil time impacts the molecular weight of the silk fi...
example 2
[0305]The present example describes a shape memory silk material with sulfonic acid modification in accordance with some embodiments of the present disclosure.
Methodology:
[0306]Cocoons of Bombyx mori were boiled for either 10 or 20 minutes in an aqueous solution of 0.02 M sodium carbonate and then rinsed with pure water. The extracted silk fibroin was dried for 12 hours in a chemical hood before being dissolved in 9.3 M LiBr solution at 60° C. for 4 hours, yielding a 20% (w / v) solution. This solution was dialyzed against distilled water using Pierce Slide-a-Lyzer cassettes, MWCO 3500 Da (Rockford, Ill.) for 3 days to remove LiBr. The solution was centrifuged to remove aggregates that formed during purification. The final concentration of aqueous silk fibroin was between 6-8% (w / v). This concentration was diluted with pure water down to 3% (w / v) for all experiments.
Preparation of Modified Silk Foams
[0307]To make silk / glycerol foams, an aqueous glycerol solution was mixed with purifie...
example 3
[0321]The present example describes a silk material that rapid re-swelling after deformation triggered by the presence of aqueous media like water or PBS in accordance with some embodiments of the present disclosure.
Materials & Methods
Preparation of Silk Solutions
[0322]Silk fibroin solution was prepared as previously reported. See for example Rockwood, D. N., Preda, R. C., YUcel, T., Wang, X., Lovett, M. L., & Kaplan, D. L., Materials fabrication from Bombyx mori silk fibroin, 6 Nature Protocols 10, 1612-31 (2011). Briefly, silk fibroin protein was extracted from Bombyx mori cocoons by boiling in a 0.02 M sodium carbonate solution for 10, 30 or 60 minutes (hereafter referred to as 10 mE (“minutes extracted”), 30 mE, and 60 mE, respectively) to remove sericin. The extracted silk fibroin was washed and dried for 12 hours in a chemical hood before being dissolved in 9.3 M LiBr solution at 60° C. for 4 hours, yielding a 20% w / v solution. This solution was dialyzed against distilled wate...
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